A chromatographic method and apparatus for serum thyroxine iodine determination including sealed ion exchanged resin column structures which are opened and press fit and sealed to funnel members for use and a method in which serum protein is removed from resin bound thyroxine in alkaline solution, with a pH of substantially 8.
A reaction cell for the performance of radioimmunoassay determinations and like saturation analysis reactions has supported within it a matrix pad of absorbent material capable of retaining the necessary reagents for the reaction and serving as a site in which the reaction totally occurs. A separable lower chamber is fitted to the lower end of the cell and contains absorbent material to abut the matrix pad and promote filtration through the pad after the reaction has taken place. An upper reservoir chamber fits to the upper end of the cell to contain liquid for passing through the matrix pad. The matrix pad will commonly contain prior to the reaction a predetermined amount of an antigen or antibody in freeze-dried condition and possibly radioactivity labelled.
