Sulfated polysaccharide, polyethylene sulfonic acid, polyvinyl sulfuric acid, polystyreme sulfonic acid, a sulfate ester of a polyethylene ether of an aliphatic alcohol having from 12 to 18 carbon atoms, monothioglycerol or sulfated naphthalene are combined with a bacterial culture medium. These compositions inhibit the antibacterial components of contaminated body fluids, thereby allowing the contaminant bacteria to grow freely.
This invention encompasses methods and reagents for inactivation of bacterial growth inhibitors present in blood, serum or plasma. It has been found that salicylates and closely related compounds will neutralize bacterial growth inhibitors present in the blood of many patients. The addition of salicylate to conventional growth medium also provides a reagent for monitoring the antibiotic levels in blood by enabling measurement of the effect of the antibiotic against a standard test organism without interference from the bacterial growth inhibitor present in sera.
This invention encompasses methods and reagents for inactivation of bacterial growth inhibitors present in blood, serum or plasma. It has been found that salicylates and closely related compounds will neutralize bacterial growth inhibitors present in the blood of many patients. The addition of salicylate to conventional growth medium also provides a reagent for monitoring the antibiotic levels in blood by enabling measurement of the effect of the antibiotic against a standard test organism without interference from the bacterial growth inhibitor present in sera.
This invention encompasses methods and reagents for inactivation of bacterial growth inhibitors present in blood, serum or plasma. It has been found that salicylates and closely related compounds will neutralize bacterial growth inhibitors present in the blood of many patients. The addition of salicylate to conventional growth medium also provides a reagent for monitoring the antibiotic levels in blood by enabling measurement of the effect of the antibiotic against a standard test organism without interference from the bacterial growth inhibitor present in sera.
In the culturing of micro-organisms, non-microbial turbidity in the supernate is prevented by including an adsorbant for components which cause such non-microbial turbidity; e.g., lipoproteins and/or chylomicrons, with such adsorbant being soluble in the culture media. The adsorbant sediments such components, and is particularly effective in culture media which include an anticoagulant. Carboxy Methyl Cellulose is a particularly effective adsorbant.
A method for increasing the quantitation of microorganisms in cell-containing or cell-free blood samples, included those intended for blood product transfusion purposes, is provided which employs a sustained level of sodium polyanethol sulfonate throughout microbial replication on solid media. In another embodiment, purified saponin is combined with sodium polyanethol sulfonate to provide increased quantitation of microorganisms.
