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Determination of nitrogenase
   
Document Number
US Patent 3904481
Issued Date
September 9, 1975
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Abstract
In a mixture of ethylene and acetylene, the ethylene is selectively oxidized to formaldehyde. This oxidation is used in the determination of nitrogenase (nitrogen fixation) activity where ethylene produced by the enzyme from added acetylene, has to be measured. The formaldehyde produced from the ethylene is proportional to the enzyme activity, and can be assayed colorimetrically or otherwise.
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Number of Claims:
6
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Published
September 9, 1975
Application Number
05/393,007
Filed
August 30, 1973
US Classification
435/4   435/167 435/191 435/808 435/810
Int'l Classification
C07C   45/00   (20060101)   C07C   45/30   (20060101)   C12Q   1/26   (20060101)  
Examiner
Attorney/Law Firm
Priority Data
Sep 15, 1972 [CA] 151822
USPTO Field of Search
195/13.5R   195/50   23/252R   23/23B  
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Claims
Description
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