A broth medium for the detection of Klebsiella, specifically Klebsiella pneumoniae and Enterobacter in urine. The medium employs 3-(.alpha.-acetonylbenzyl)-4-hydroxycoumarin and 2,4 dinitro-phenyl hydrazone of .alpha.-Ketoglutoric acid to inhibit growth of Escherichia Coli organisms and to promote growth of Klebsiella. This medium also has a pH of about 8.0 to inhibit other organisms which grow under more neutral conditions.
The sensitivity of Klebsiella and Enterobacter organisms to preselected antibiotics is determined with a medium consisting essentially of a nutrient source for Klebsiella and Enterobacter organisms, about 0.01 to about 0.03 g/l 2,4 dinitro phenyl hydrazone of .alpha.-Ketoglutaric acid, about 3.5 to about 6 g/l sodium desoxycholate and the preselected antibiotic.
2-Deoxy-D-Ribose is used as a carbohydrate source which selectively differentiates all of the Salmonella spp., Arizona spp., and 60% of the Citrobacter freundii from other members of the Enterobacteriaceae family. By combining the 2-Deoxy-D-Ribose with Citrobacter freundii inhibitors and thioglycollic acid, sodium salt to impart semi-anaerobic conditions in the medium as well as suitable nutrients and buffers, a selective Salmonella broth is compounded which lends itself to automated identification or enumeration systems.
