A novel antigenic preparation which comprises a plurality of microvesicles are unilamellar bodies comprising a single lipid bilayer upon the exterior surface of which is bound an antigenic protein derived from a virus and having a hydrophobic region.
The outer membrane of influenza virus is attached to a liposome by two different techniques. In addition, one of the techniques allows the entrapment of intact virus, usually one virus per liposome. The techniques can be performed with either influenza virus A or B.
A novel antigenic preparation which comprises a plurality of microvesicles which microvesicles are unilamellar bodies comprising a single lipid bilayer upon the exterior surface of which is bound an antigenic protein derived from a virus and having a hydrophobic region.
Disclosed are immunostimulating reconstituted influenza virosomes (IRIVs) comprising reconstituted functional virus envelopes containing an inactivated hepatitis A virus and an influenza hemagglutinin protein (HA) or a peptide with the amino terminal 21 amino acid residue segment of HA.sub.2 which induces the fusion of the IRIVs with cellular membranes and the lysis of the IRIV. A vaccine containing the hepatitis A IRIVs is also disclosed.
Subunit viral or bacterial antigens are incorporated into liposomes containing a positively charged amino-containing surfactant. The resulting complex is antigenically more active than the free antigen.
The present invention relates to novel fusogenic vesicles as highly efficient and versatile encapsulation systems for delivering a substance of choice, such as nucleic acids, proteins, peptides, antigens, pharmaceutical drugs and cosmetic agents to cells and tissues.
