An apparatus for effecting mass transfer in fermentation reactions is disclosed. The apparatus includes a cylindrical vessel with two impellers located toward the top and bottom of said vessel, one of which is an axial flow impeller and the other is a radial flow impeller. A draft tube of uniform diameter throughout substantially all of its length having an open bottom is located within and coaxial with said cylindrical vessel. The lower impeller being said radial flow impeller is located below the lower end of the draft tube.
Here is disclosed a cell culture tank comprising a cylindrical screen mounted upright within the cell culture tank and having upper and lower openings through which culture medium flows, a pipe for supply of oxygen or oxygen containing gas and an agitator adapted to produce a flow of the culture medium, both the pipe and the agitator being provided within the cylindrical screen, wherein the agitator drives the culture medium to flow downward through the lower opening of the cylindrical screen, then to flow upward into a space defined between the cylindrical screen and the inner wall of the cell culture tank, and a relationship between an ascending velocity of the culture medium and a sinking velocity of the adherent carriers produces in the space between the cylindrical screen and the inner wall of the cell culture tank a floating region within which the adherent carriers float in the culture medium and a separating region within which the adherent carriers are separated from the culture medium.
A method for the production of a bacterial cellulose by cultivating a cellulose-producing bacteria for at least a certain period of time under such an apparatus condition that an oxygen-transfer coefficient (K.sub.L a) is about 25 to about 50/hr, said K.sub.L a is determined by using a simulated suspension containing 2% by weight of BC and having a plastic viscosity of 15 to 20 poise.
Fat or oil is hydrolyzed with water and lipase effectively by supplying the fat or oil and water continuously each at a constant rate or semicontinuously in portions and simultaneously withdrawing a solution containing fatty acid(s) and an aqueous solution containing glycerol formed by the enzymatic reaction from the reaction system continuously at the same rates as those of the supplied fat or oil and water respectively or semicontinuously in portions to thereby maintain the glycerol concentration in the aqueous phase of the reaction system constant within a range of 10 to 40% by weight.
5395593 - Processing vessel - Owned by The Secretary of State for United Kingdom Atomic Energy Authority in Her and Northern Ireland (Didcot,GB)
A processing vessel (10) for a liquid includes an impeller (32) in an open-ended shroud (30) to cause circulation. The base of the vessel (10) communicates with a short duct (18) closed at its lower end, and the shroud (30) is shaped to ensure circulation into this dead-end duct (18). The duct is provided with ultrasonic modules (24) so that its contents are subjected to intense insonation. The insonation of the liquid can lead to improvements in the product of processes such as crystallization.
