A process for differential diagnosis of various rheumatological and arthritic diseases, all of which may yield similar antinuclear antibody test results. The process comprises a continuous sequence of tests on a single serum specimen to minimize and eliminate variations, thus providing accurate results. The process involves drawing a serum from a patient and first performing an antinuclear antibody test which is evaluated. If the evaluation is positive, then the particular type of positive indication is interpreted. If the interpretation reveals peripheral or speckled immunofluorescence, then a diagnosis of active Systemic Lupus Erythematosus (SLE), or mixed connective tissue disease (MCTD) are suggested, and automatically additional confirming tests will be performed as part of the process. These tests are the anti-DNA (Deoxyribonucleic Acid), the anti-ENA (extractable nuclear antigen). The test for the complement component C3 and a muscle disease test, called a creatine phosphokinase (CPK) which is a test for an enzyme contained in skeletal muscle. The results are compiled and reported in addition to reporting the degree of positivity by titer, which is the strength of a solution or the concentration of the antinuclear antibodies determined by a series of titrations or dilutions.
An AC split-phase induction motor has a two-pole run winding, a four-pole run winding and a separate start or phase winding. The two-pole winding is connected directly to a corresponding first power terminal. The second run winding is connected to a second power terminal by a solid state gated run switch having a turn-on gate. The start winding is connected in circuit in series with a solid state gated start switch connected to the first power terminal and having a gate connected to a second power terminal. A centrifugal switch connects the first and second power terminals. The gate of the run switch is connected to the start winding circuit and includes connecting Diac and resistance elements to actuate the run switch only in response to a power connection to the second power terminal and the opening of the centrifugal switch. In operation, the motor always starts with the two-pole run winding and the start winding in the circuit. At switching speed, the start switch disconnects the start winding. Depending upon the power terminal connection, the motor continues to run with power supplied to the two-pole run winding or actuates the solid state gated run switch of the four-pole winding to operate as a four-pole motor.
A method for diagnosis of rheumatoid arthritis and related diseases comprises determination of polyclonal lymphocyte activation in B-cells cultured in the presence of patient serum. A diagnostic kit is also provided.
Targeting protein-diagnostic/therapeutic agent conjugates joined by stabilized Schiff base linkages are disclosed. Schiff base linkage of targeting protein and agent is accomplished without exposure of the targeting protein to harsh oxidizing or reducing conditions. The cleavable, heterobifunctional linkers that are described provide certain advantages relating to in vivo administration of targeting protein conjugates, including controlled release of active agent at a target site.
Targeting substance-diagnostic/therapeutic agent conjugates joined by stabilized Schiff base or hydrazone linkages are disclosed. In addition, slow release carrier-drug pharmaceuticals are described. The diagnostic and therapeutic conjugates and pharmaceuticals of the present invention provide certain advantages relating to in vivo administration, including controlled release of the active agent at a target site.
