An oxalate oxidase can be extracted from the stems of beet by homogenizing the beet, filtering, centrifuging the filtrate, precipitating the oxalate oxidase containing fraction with acetone, dissolving the precipitate in taurodeoxycholic acid, dialyzing the solution and column chromatography. Unlike prior art oxalate oxidase compositions, the instant composition is relatively insensitive to sodium chloride and is well adapted to rapid and inexpensive oxalate assay in body fluids. The composition is inactive on almost all body fluid components other than oxalate, the only significant interfering component being ascorbate, and this ascorbate is readily removed by treatment with acid ferric chloride prior to the oxalate oxidase assay procedure, the ferric ion being thereafter removed by a cation exchange resin.
The subject invention pertains to a novel assay device for detecting oxalate in a sample. The assay device comprises enzyme and dye compositions immobilized on a solid carrier matrix. The subject invention can also be used to measure the concentration of oxalate in a sample. The subject invention further pertains to a novel oxalate oxidase composition and methods of preparing the subject enzyme composition. The oxalate oxidase composition can be used in the assay device of the subject invention.
The subject invention concerns the novel use of formyl-CoA transferase enzyme together with oxalyl-CoA decarboxylase enzyme for the detection and measurement of oxalate in biological samples. The use of the enzyme system according to the subject invention results in the conversion of oxalate into carbon dioxide and formate. Because the production of formate is directly correlated to the concentration of oxalate present in a sample, the determination of the resulting formate concentration provides an accurate, sensitive and rapid means for detecting even low levels of oxalate. The subject invention further concerns the cloning, sequencing and expression of the genes that encode the formyl-CoA transferase enzyme and the oxalyl-CoA decarboxylase enzyme of Oxalobacter formigenes. The subject invention also concerns a method for detecting the presence of Oxalobacter formigenes organisms in a sample, and the polynucleotide probes and primers used in the detection method.
The invention provides a process for producing mixtures of glyoxylic acid and aminomethylphosphonic acid. The process comprises reacting glycolic acid and oxygen in an aqueous solution in the presence of aminomethylphosphonic acid catalyst consisting of glycolate oxidase and catalase. The resulting mixtures are useful intermediates in the production of N-(phosphonomethyl)glycine.
The subject invention concerns the novel use of formyl-CoA transferase enzyme together with oxalyl-CoA decarboxylase enzyme for the detection and measurement of oxalate in biological samples. The use of the enzyme system according to the subject invention results in the conversion of oxalate into carbon dioxide and formate. Because the production of formate is directly correlated to the concentration of oxalate present in a sample, the determination of the resulting formate concentration provides an accurate, sensitive and rapid means for detecting even low levels of oxalate. The subject invention further concerns the cloning, sequencing and expression of the genes that encode the formyl-CoA transferase enzyme and the oxalyl-CoA decarboxylase enzyme of Oxalobacter formigenes. The subject invention also concerns methods for detecting the presence of Oxalobacter formigenes organisms in a sample, and the polynucleotide probes and primers used in the detection method.
A single color determination method of quantization of the amount of fructosamine in a sample such as serum by removing other interfering reducing agents and developing a color with a coloring agent such as tetrazolium salt.
