A micro-titre test plate includes a thermoplastic incubation tray having an array of a plurality of wells extending therethrough, a filter, and a thermoplastic harvester tray for supporting the filter sheet. The harvester tray has a plurality of apertures therethrough arranged to be registerable with the bottoms of the wells in the incubation tray. To prevent cross-talk between the wells along the filter, the facing surfaces of the harvester tray and incubation tray are provided with mating ridges and grooves circumscribing the juncture of each well and aperture. In one embodiment, the ridges and grooves are thermally bonded to one another to provide a fluid-impervious barrier trapping respective filter disks in the juncture of each well and aperture. In another embodiment, the harvester tray is formed of a pair of apertured, thermoplastic sheets nested with respective thermally bonded grooves and ridges to trap the filter, the harvester tray being not necessarily bonded to the incubation tray.

A cover plate, having a lower, flat surface containing an array of numerous, parallel, closely spaced apart, downwardly opening channels, is releasably clamped upon a flat support plate surface. The channels each have inlet and outlet holes communicating with the upper surface of the cover plate. A paper-like, resilient cushion sheet is clamped between the plate surfaces and a paper-like membrane, carrying a pattern of antigen-like material, is arranged between the sheet and the cover plate channeled surface. The channels are very narrow, shallow and many times longer than their widths and are closed by the stripe-like portions of the membrane which overlie the channels. The cushion sheet is compressed between adjacent channels and bulges upwardly at each of the channels to thereby bulge the membrane stripe-like portions into dome-shaped cross-sections extending into the channels. This reduces the volumes of the channels and increases the surface areas of the membrane stripe-like portions that are exposed within the channels. Numerous drop-size samples of antibody-type solutions are inserted into the channels through channel inlet holes, that extend through the cover plate, for reacting with the material carried on the stripe-like portions of the membrane. A removable manifold having a pair of elongated grooves may be temporarily applied upon the cover plate so that the grooves overlie all of the channel inlet holes and all of the channel outlet holes for rapidly, simultaneously filling and draining all the channels.

This invention relates to a device for detecting on a nitrocellulose-type sheet the presence of macro-molecular complexes, having: a bottom adapted to receive said nitrocellulose sheet, a plate adapted to rest on said nitrocellulose sheet applied on the bottom and having a plurality of parallel grooves that pass through said plate over the whole of its thickness and over the whole length of said grooves.

A method and device for the simultaneous production of chemical compounds in an array which is capable of providing a very broad range of reaction environments including reaction temperatures of -40.degree. C. to 150.degree. C., reflux condensation, and a selective gas environment. The invention also allows the addition of several reagents during the course of the production process. The device is comprised of a number of different block sections which are fastened together to provide the required reaction environment.

A sealing member for a multi-well microfiltration plate comprises a flexible sealing material which, in response to the application of differential pressure, will flex or collapse in the direction of the filtration along the contour of each individual well. A process maintains a differential pressure substantially constant over a multi-well microfiltration plate comprising placing said flexible sealing member over that surface of the plate having well openings, stretching the flexible sealing member so as to seal the perimeter of each individual well, creating a differential pressure around said plate covered with said seal and filtering a media from each occupied well at a rate independent of the filtration rate of any other well while maintaining a substantially constant differential pressure around the plate until filtration in the last well containing media is complete.