Method of binding nucleic acid to a nucleic acid-binding support comprising depositing the nucleic acid onto the support and then contacting the nucleic acid and the support with a liquid binding solution which is compatible with the support and which contains an organic solvent which is capable of binding the DNA to the support, for a period of time sufficient to effect the binding.
A process for solid phase sequencing of nucleic acid fragments is disclosed. The object of the invention is to provide a sequencing process which enables the simultaneous sequencing of large amounts of long and short nucleic acid fragments and which is optionally automated. Thus, a solid support combining mechanical stability, anion exchange characteristics, and chemical elution of nucleic acids off the support is used. Immobilized nucleic acid fragments are chemically modified and subsequently the nucleic acid backbone is cleaved and simultaneously or subsequently said fragments are eluted by chemical means. The present invention is applied to molecular biology and gene technology.
A method for binding a target polynucleotide in a sample is provided. The method uses an assay reagent which is a substrate noncovalently bound to a hydrophobic moiety which is part of a polynucleotide capture probe. This assay reagent is contacted with the sample under conditions which permit hybridization between said capture probe and said target polynucleotide, thereby binding the target polynucleotide.
This invention features vectors and a method for sequencing DNA. The method includes the steps of: (a) ligating the DNA into a vector comprising a tag sequence, the tag sequence includes at least 15 bases, wherein the tag sequence will not hybridize to the DNA under stringent hybridization conditions and is unique in the vector, to form a hybrid vector, (b) treating the hybrid vector in a plurality of vessels to produce fragments comprising the tag sequence, wherein the fragments differ in length and terminate at a fixed known base or bases, wherein the fixed known base or bases differs in each vessel, (c) separating the fragments from each vessel according to their size, (d) hybridizing the fragments with an oligonucleotide able to hybridize specifically with the tag sequence, and (e) detecting the pattern of hybridization of the tag sequence, wherein the pattern reflects the nucleotide sequence of the DNA.
A polynucleotide which has been modified by a hydrophobic moiety so that the polynucleotide attaches to a substrate by the hydrophobic moiety, rather than by a portion of the polynucleotide, thereby allowing the polynucleotide to be available for hybridization.
An assay article for detection biopolymers contained in a sample is described. The assay article includes a substrate and a biopolymer directly adsorbed on the surface of the substrate. A plurality of biopolymers may be adsorbed on the surface of the substrate to form an array. Also disclosed is a method of making the assay article. In the preferred method, an aminated polypropylene substrate is used. A biopolymer is contacted with the aminated substrate under a condition sufficient for direct adsorption of the biopolymer on the surface of the substrate. A method of detecting a target biopolymer contained in a sample is also disclosed. In this method, a substrate is contacted with either a probe or target biopolymer under a condition sufficient for a direct adsorption of either the probe or target biopolymer on the substrate to form a probe assay article or a target assay article. Then, the probe assay article is contacted with the target biopolymer, or the target assay article is contacted with the probe biopolymer under a condition that allows the formation of a probe-target complex. Finally, the complex is detected and the presence of the complex is used as a measurement for the presence or the amount of the biopolymer target contained in the sample.
