An emulsion (in which an aqueous medium contains small liquid droplets coated with a protein that will interact specifically with a select protein) is mixed with a liquid sample, time is allowed for interaction to occur and the mixture is then exposed to a tagged antibody specific to the select protein. After an appropriate hold time the emulsion is broken and the protein that previously covered the disperse droplets becomes concentrated at the surface. The surface is checked for the presence of the tagged antibodies to establish the presence of absence of the select protein.
A pharmaceutical composition comprising an oil/water emulsion wherein the oil droplets contain a drug in dissolved or dispersed or solubilized form. The droplets are further coated with adsorbed native or modified antibodies which provide targeting of the droplets and the drug. The process for preparing this composition comprises the steps of (i) dissolving or dispersing a drug in an oil phase, (ii) preparing an oil/water emulsion, (iii) obtaining surface-active antibodies by chemical or physical attachment of hydrophobic groups to the antibodies, and (iv) mixing the surface-active antibody with the emulsion.
Bioaffinity and ion-exchange separation methods are provided along with liquid supports utilized in these methods. The support is based on an inert carrier with ligands or binders attached to its surface through a highly fluorinated isocyanate anchor group. Methods for preparing such supports and their use in capturing neutral and charged target molecules from samples and in analytical applications are also provided.
Liquid perfluorocarbon supports useful as liquid affinity supports are provided. The support is based on an inert perfluorocarbon carrier with ligands or binders for the ligands attached to its surface through a highly fluorinated isocyanate anchor group.
Immunoassays and nucleic acid assays are provided along with liquid perfluorocarbon supports utilized in these methods. The support is based on an inert perfluorocarbon carrier with ligands or binders for the ligands attached to its surface through a highly fluorinated isocyanate anchor group.
Methods for preparing liquid supports utilized in bioaffinity and ion-exchange separation methods and provided. The support is based on an inert carrier with ligands or binders for ligands attached through its surface through a highly fluorinated isocyanate anchor group. The use of such supports in capturing neutral and charged target molecules from samples and in analytical applications are also provided.
