Process for solubilization, purification and characterization of protein from insoluble protein aggregates or complexes and compositions of matter therefrom
This invention discloses a novel process for the solubilization, purification and characterization of a protein or proteins from insoluble protein aggregates or complexes. The novel process comprises the use of a dissociating step gradient which can be followed by further purification and concentration. Also disclosed are compositions of matter and vaccines comprising one or more proteins purified according to the novel process of this invention.
A method for the recovery of proteins which method includes providing a source of protein in an insoluble form, a source of at least one cationic surfactant; treating the insoluble protein with the at least one cationic surfactant in an amount sufficient to effect solubilization without substantial modification to the structural backbone of the protein.
Lewis acid-Lewis base high molecular weight salt microparticulate material of the type referred to in U.S. Pat. No. 3,959,457, which include biologically active peptides and proteins solubilized in a non-aqueous, non-denaturing manufacturing solvent.
A method of fractionating components separated in liquid chromatography. The method comprises the steps of applying a sample to a column filled with a separation media while supplying an eluent to the column, causing eluent portions containing components in the sample to flow into a fractionation tube connected to the end of an eluent flow path, holding the eluent portions in the fractionation tube, and injecting air into a rear portion of the eluent portions in the fractionation tube.
A method for the renaturation of unfolded proteins comprises reversibly immobilizing the denatured protein on a solid phase and inducing folding of the immobilized protein by progressively reducing with time the concentration of a denaturing agent in the solvent in contact with the solid phase. The refolded protein is recovered from the solid phase in native form. The proteins can be folded and recovered in high yield in a small volume of buffer.
Apparatus for fractionating components separated in liquid chromatography is disclosed. The apparatus includes a sample applying section, a column section, a detector section, and a tubing line connecting the sections. The apparatus further includes a fractionation tube for fractionating eluent portions containing prospective components separated from the column, an air injector for injecting air into the fractionation tube, and a valve for a switching operation such that either of the eluent fed through a flow path or air fed through an air flow path from the air injector is led into the fractionation tube while the other is drained to the drain side.
