The regeneration of plants from cotton callus (Gossypium hirsutum L. cv Coker 310) is described. Somatic proembryoids developed spontaneously after two years in culture on a modified Linsmaier and Skoog medium. The percentage of calli forming proembryoids was increased to about 30% by prolonged culture without NAA and kinetin. Development of proembryoids was enhanced by transferring proembryoids to media lacking NH.sub.4 NO.sub.3 but containing double the standard KNO.sub.3 concentration and gibberellic acid. Root initiation and growth was promoted by lowering the glucose concentration to 5 g/l.
A method for producing a stigma-like tissue of a saffron flower comprising (1) culturing a corolla tube of a saffron in the presence of a plant hormone or (2) culturing a female organ and/or a corolla tube of a saffron flower in the presence of a plant hormone and alanine, and a method for producing useful components of a saffron flower, e.g., saffron pigment, from the thus produced stigma-like tissue. The tissue cultures produce larger quantitites of the stigma-like tissue and useful components, compared to that obtained by conventional tissue cultures.
Disclosed herein is a process for breeding a glabrous variety of rice crop, this process comprising the steps of culturing tissues from a target rice plant, culturing the calli formed to a regeneration medium, growing the regenerates and selecting glabrous plants therefrom them, a new and distinct variety of a glabrous variety of a rice plant obtained by this process, and a process for the production of a glabrous plant which comprises further reproducing the plants. This glabrous variety is useful for rice cultivation.
A method is disclosed for producing a transgenic cotton plant comprising the steps of (a) obtaining cottonfibrous root explants, (b) culturing the fibrous root explants to induce callus formation, (c) exposing root callus to a culture of Agrobacterium tumefaciens that harbors a vector comprising an exogenous gene and a selectable marker, the Agrobacterium being capable of effecting the stable transfer of the exogenous gene and selection agent resistance gene to the genome of the cells of the explant, (d) culturing the callus in the presence of the selection agent to which the selection agent resistance gene confers resistance so as to select for transformed cells, (e) inducing somatic embryo formation in the selected callus culture, and (f) regenerating the induced somatic embryos into whole transgenic cotton plants.
Hybrids of onion and garlic or Chinese chive are obtained by culturing the ovule-embryo after fertilization of the male (e.g. garlic pollen) and female (e.g. onion egg cell) gametes. The hybrids have the useful characteristics of both species and can be propagated.
The present invention discloses a process for transforming mature trees of Eucalyptus plants comprising: induction adventitious shoots from segments of the explant obtained from an adult tree of a Eucalyptus plant, preculturing the adventitious shoots in infection induction medium, infecting the adventitious shoots subjected to infection induction treatment with infection medium containing Agrobacterium tumefaciens, and rotary-culturing the infected explant segments in sterilization medium containing antibiotic; whereby sterilizing and forming transgenic calli, which regenerate transgenic plants by way of formation of shoot primordia by rotary-culturing under illumination.
