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BACKGROUND OF THE INVENTION
Frequency domain cross-correlation fluorometry is a known process by which
excited light is modulated at a given frequency. The phase shift and the
modulation ratio of emission with respect to excitation are measured to
obtain the lifetime of the excited state, making use of the
cross-correlation technique. This is a versatile tool for determining many
subtle characteristics of matter and their interactions. See, for example,
the article by E. Gratton and B. Barbieri entitled Multifrequency Phase
Fluorometry Using Pulsed Sources: Theory and Applications; Spectostropy,
Vol. I, No. 6, pp. 28-38 (1986). See also the article by Gratton, Jameson
& Hall entitled Multifrequency Phase and Modulation Fluorometry; Ann. Rev.
Biophys. Bioeng. Vol. 13, pp. 105-124 (1984). See also the article by
Gratton and Limkeman entitled A Continously Variable Frequency
Cross-Correlation Phase Fluorometer With Picosecond Resolution,
Biophysical Journal, Vol. 44, pp. 315, 324 (1983). The above articles are
given by way of example only, with other articles being available as well.
Fluorometers having continuous frequency domain cross-correlation
fluorometric function are commercially available from I.S.S. Inc. of
Champaign, Ill., such fluorometers being sold under the trademark GREG.
Such a device is capable of measuring and analyzing excitation and
emission spectra, fluorescence decays, phase and modulation resolved
spectra, time-resolved spectra, and the dynamic depolarization of various
materials. As is well known, the apparatus has a highly collimated xenon
arc lamp for steady-state measurements and routine lifetime
determinations, plus a laser source (UV-visible) for lifetime measurements
requiring extremely high sensitivity and accuracy. The modulation of the
excitation light is obtained using a wide band electro-optical modulator,
specifically a Pockels cell. The Pockels cell is modulated by a direct
synthesis frequency synthesizer which provides a signal to the Pockels
cell at a first frequency. Another direct synthesis synthesizer provides a
signal, in phase coherence with the first synthesizer by driving the two
synthesizers from the same quartz crystal. This second synthesizer
provides a signal at a second frequency, different from the first
frequency, to a pair of detector units set up for detecting luminescence
in the sample generated by the modulated light source.
The signal at the output of the detector unit contains a component which is
of a frequency that is the difference between the two frequencies, which
difference is named the "cross-correlation frequency". The
cross-correlation frequency is filtered and processed by a data
acquisition unit to provide both the phase shift and the modulation
difference of the luminescence of the sample, when compared with the
modulated excitation light directed at the sample.
The electronic circuits are designed to filter and process the
cross-correlation frequency component only. At the present time, only
cross-correlation frequencies of 25 hertz, 31 hertz, and 40 hertz are
used. Appropriate phase coherence at these low frequencies are obtained by
use of the direct synthesis frequency synthesizers which have a resolution
of 1 hertz.
It would be desirable to use phase-locked loop frequency synthesizers
because their cost per unit is currently at least two thousand dollars
less than each direct synthesis synthesizer unit. However, their use has
been, up to the present time, impractical due to the phase noise of the
cross-correlation frequencies used in the prior art.
In accordance with this invention, apparatus for frequency domain
cross-correlation fluorometry is provided which utilizes phase locked-loop
frequency synthesizers. For this reason alone, the cost saving in the
apparatus, when compared with the use of direct synthesis synthesizers,
may currently be at least four thousand dollars per unit and very probably
more than that. Furthermore, phase-locked loop synthesizers operate in a
larger frequency range, which increases the lifetime range measurable
using cross-correlation frequency domain fluorometers. Moreover, the
electronic filter that separates the cross-correlation frequency component
of the output signal is cheaper than the corresponding electronics
involved with the direct synthesis synthesizers, and is of easier
construction. Additionally, the improvements of this invention allow
faster measurements, with reduced dead time between two consecutive
measurements. This, in turn, greatly facilitates the possibilities in
studies of lifetime kinetics measurements.
DESCRIPTION OF THE INVENTION
In accordance with this invention, apparatus for frequency domain
cross-correlation fluorometry is provided, which comprises: a source of
electromagnetic radiation and means for amplitude modulating the
electromagnetic radiation at a first frequency. Means are also provided
for directing the amplitude-modulated electromagnetic radiation at a
sample to cause the sample to respond by luminescence (typically
fluorescence), plus means for detecting such luminescence.
A signal is also provided to modulate the gain of the detecting means,
which signal is coherent with the amplitude modulating means and is at a
second frequency, different from the first frequency. Preferably, both the
amplitude modulating means and the means for providing the signal at the
second frequency each comprise a phase-locked loop frequency synthesizer.
The synthesizers operate coherently with each other, typically off of a
single quartz crystal in one of the synthesizers, with the other
synthesizer acting in slave relationship to the first synthesizer.
In accordance with this invention, the second frequency is different from
the first frequency by at least 100 hertz, a significant increase over the
frequency difference used in apparatus of the prior art. Specifically, it
is preferred for the signal difference to be from 500 to 2,000 hertz, and
generally no more than 5000 hertz.
Means are also provided for deriving a resultant signal from the
electromagnetic radiation and the detecting means at a frequency of the
difference between the first and second frequencies. This resultant signal
may be processed through conventional electronics and a computer, in a
manner similar to the prior art apparatus, to detect phase shift and
modulation changes of the luminescence when compared with the
electromagnetic radiation. Typically, the detecting means is a
photomultiplier, having the second frequency imposed upon it by one of the
phase-locked loop frequency synthesizers, to modulate the gain of the
photomultiplier at the second frequency. The resulting product of the
signal of the first frequency, and of the gain of the detecting means at
the second frequency, provides a component at a frequency that is the
difference between the two frequencies (the cross-correlation frequency)
which is filtered by conventional electronics to measure phase shift of
the emission and the modulation of the emission; specifically, the ratio
between the modulation of the emission and the modulation of the
electromagnetic radiation used to produce the emission.
By this technique, deep insights into the structure and interactions of
matter can be obtained.
Phase-locked loop frequency synthesizers are well known to the art. See,
for example, the fifth edition of the book by Robert L. Schrader entitled
Electronic Communication, pp. 222-224. Basically, in a phase-locked loop
frequency synthesizer, a stable (and typically crystal) reference
oscillator AC, and the AC from an oscillator having a frequency which can
be varied by a DC voltage applied to it, are both fed to a phase detector
circuit. If the frequency and phase of the reference and
voltage-controlled oscillator signals are equal, the DC output voltage
from the phase detector will be some particular value. This DC is fed
through a low pass filter to a DC amplifier and to the voltage input
circuit of the voltage controlled oscillator. If the voltage-controlled
oscillator tries to shift off frequency, the phase detector develops a
change in the DC "error" voltage which corrects the voltage-controlled
oscillator frequency and locks it to the reference frequency again. The
voltage-controlled oscillator frequency is corrected not only to exactly
the frequency of the reference AC, but to the same phase. One locked in,
changes of the reference oscillator frequency are tracked by the
voltage-controlled oscillator units. Further, well-known details of the
phase-locked loop frequency synthesizer are available in the previously
cited reference and elsewhere. Phase-locked loop synthesizers are
available, for example, from the Syntest Corp. of Marlboro, Mass., or
Marconi Instruments of Allendale, N.J.
DESCRIPTION OF DRAWINGS
In the drawings,
FIG. 1 is a diagrammatic view of a cross-correlation frequency domain
fluorometer, improved in accordance with this invention.
FIG. 2 is a flow diagram of the analog electronics of the device of FIG. 1.
DESCRIPTION OF SPECIFIC EMBODIMENT
Referring to the drawings, the apparatus of this invention may constitute,
for example, a GREG 200 multifrequency cross-correlation phase and
modulation fluorometer sold by ISS Inc. of Champaign, Ill., with only
those modifications as described herein. Alternatively, other designs of
fluorometry machines may use the invention of this application as well.
Cross correlation frequency domain fluorometer 10 may comprise four major
components: intensity-modulated light source unit 12, frequency generator
unit 14, optical module 16 including the sample holder and the light
detectors, specifically the photomultiplier units, and data acquisition
unit 18.
A light source of unit 12 may be a laser 20, for example a Liconix 4207 NB
He-Cd laser. A xenon lamp 22 is provided as an alternate light source in
the specific apparatus shown. Alternatively, synchroton radiation or
mode-locked lasers may be used for studies of high criticality.
Laser light is reflected from mirror 24, or, alternatively, the output of
arc lamp 22 is focused through lens 26 and reflected off of mirror 28, to
cause light beam 30 to pass through two-way polarizer 32 and through
Pockels cell 34. If laser 20 is used, mirror 28 is removed.
Two-way polarizer 32 may be of the Glan-Taylor type, while the Pockels cell
may be a LASERMETRICS 1042 Pockels cell. Pockels cell 34 may be controlled
by power unit 36.
Light beam 30 passing through Pockels cell 34 is reflected from mirror 38
back through the Pockels cell, being deflected by two-way polarizer 32
along light path 40, through electronic shutter 42, lens 44, and motor
driven monochromator 46, so that emergent light beam 48 may be of
essentially a single color or wavelength, typically in the near
ultraviolet or visible (180 nanometers to 800 nanometers). The selection
of the wavelength depends, of course, upon the absorbancy of the material
to be studied.
Frequency unit 14 comprises a pair of phase-locked loop frequency
synthesizers 50, 52. Synthesizers 50, 52 are electrically connected by
lead 54, and conventionally driven in coherent manner off the same quartz
crystal positioned in one of the synthesizers, to produce first and second
signals having a frequency difference of, in this specific case, one
kilohertz.
Synthesizer 50 provides an output frequency through conductor 56 to Pockels
cell 34, to impose upon the light beam passing through it an amplitude
modulation at a corresponding frequency. In the particular apparatus
shown, the frequency is highly variable and adjustable, typically ranging
from 10 kilohertz to 1000 megahertz, and higher as desired, this frequency
corresponding to the first frequency described above.
Thus, light beam 48 is both amplitude modulated and monochromatic after
leaving monochromator 46. It passes through lens 58 and beam splitter 60,
to provide a pair of beams. One beam 62 passes through filter 64, when
such is desired, shutter 66, and lens 68, to impinge reference detector
70. This and all the detectors present may be Hamamatsu R928
photomultipliers.
The other split beam 71 passes through a corresponding filter 64a, shutter
66a, Glan-Taylor polarizer 72, and lens 74, to impinge upon a sample in
sample holder 76. There, the fluorescence or luminescence of the sample
material is elicited in conventional manner by irradiation. The resultant
fluorescence may be directed to a pair of emission detectors 78 along a
pair of different beam paths, each path including, respectively, lenses
80, manual shutters 82, optional filters 84, and Glan-Taylor polarizers
86. One of the beam paths, as shown, may include a second motor driven
monochromator 88.
Second frequency synthesizer 52 provides a signal at a second frequency
through conductors 89 to modulate the gain of detectors 78. The second
frequency is, specifically, one kilohertz apart from (either greater or
less) the first frequency from synthesizer 50, with both frequencies being
adjustable together while maintaining their one kilohertz difference.
The processing electronics 18 are typically formed on a single card, and
are connected to reference detector 70 through conductor 90 and to each of
detectors 78 through conductors 92 and 92a.
As shown in FIG. 2, processing electronics 18 include two identical
channels: one for the signal from reference detector 70 through conductor
90, and the other for the signal from the detector 78 which detects the
fluorescence through conductor 92 and 92a. If desired, a third independent
channel for one of the detectors 78 may be provided, but it is generally
contemplated to use the detectors in alternative manner, or with their
outputs through conductors 92, 92a connected together.
The signal coming into the input of each channel has an AC and a DC
component, in view of the frequencies imposed on the various signals in
the system by frequency synthesizers 50, 52. The signal passes through
current to voltage converter 93, 93a, with the AC component of the signal
being fed through capacitors 94, 94a, and then through a 1 kilohertz
filter 96, 96a (typically A.P. Circuit Corp., New York City). The signal
is then fed to an RMS converter 98, 98a, and the input to RMS converter
98, 98a is connected to a zero crossing detector 100, 100a. The signal
from RMS converter 98, 98a is fed through amplifiers 102, 102a and to
voltmeter 104, 104a. The signal from zero-crossing detector 100, 100a
forms a square wave for phase measurement.
Simultaneously, the direct current component is fed through DC integrator
106, 106a, and to direct current voltmeter 108, 108a. The four digital
voltmeters 104, 104a, 108, 108a, are provided for continuous monitoring
of the DC and AC parts of the signals from the respective photomultipliers
70, 78. The results of this monitoring are conveyed to computer and
peripherals 110, typically an Apple 2E or IBM PC personal computer with
dual disc drive and graphic printer. Commercially available programs can
provide interpretation of the data received for analysis of a wide variety
of physical phenomena. The use of a typically one kilohertz filter for
filters 96, 96a, corresponds with a one kilohertz difference in the
frequency of the signals emitted by synthesizers 50, 52.
As the result of this, it becomes possible to use phase-locked loop
frequency synthesizers as generators of the necessary phase-coherent
signals for cross-correlation frequency domain fluorometry. Currently,
savings of at least four thousand dollars per unit can be achieved over
prior designs by the replacement of the direct synthesis synthesizers of
the prior art in this unit. It can be seen that synthesizers 50, 52 may be
operated at other differential frequencies than 1 kilohertz as described
above, in that circumstance, filters 96, 96a will be correspondingly
modified.
The above has been offered for illustrative purposes only, and is not
intended to limit the scope of the invention of this application, which is
as defined in the claims below.
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