Biologically active lymphotoxin polypeptide species, and derivatives, fragments, aggregates and pharmaceutically acceptable salts are provided. The lymphotoxins are substantially homogenous, and are formulated into pharmaceutical compositions. The lymphotoxins are purified to a specific activity of at least 10.sup.6 units/mg protein by using hydrophobic substances and/or immobilized lentil lectin, or with the use of other chromatographic processes such as ion exchange chromatography, HPLC or gel filtration.
This is a continuation of application Ser. No. 608,316, filed May 7, 1984, now abandoned, which is a continuation-in-part of U.S. Ser. No. 06/403,671 filed July 30, 1982 now abandoned.
Peptides which consist of 4-25 amino acids and which bind to tumor necrosis factor-alpha, prevent tumor necrosis factor-alpha from binding to its receptors and inhibit tumor necrosis factor-alpha activity are disclosed. Methods of inhibiting tumor necrosis factor-alpha activity and of treating individuals suffering from tumor necrosis factor-alpha-mediated diseases and disorders are disclosed.
Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin-A or a mixture thereof, the resulting cells are fractionated by sucrose density-gradient centrifugation method to isolate a messenger RNA in 12.6S to 14.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the base sequence of the Table 1 as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.
Peptides which consist of six to eight, predominately D-amino acids and which bind to tumor necrosis factor-alpha, prevent tumor necrosis factor-alpha from binding to its receptors and inhibit tumor necrosis factor-alpha activity are disclosed. Methods of inhibiting tumor necrosis factor-alpha activity and of treating individuals suffering from tumor necrosis factor-alpha-mediated diseases and disorders are disclosed.
5095096 - Fused protein comprising lymphotoxin - Owned by Sagami Chemical Research Center (Tokyo,JP) Central Glass Company, Ltd. (Yamaguchi,JP) Hodogaya Chemical Co., Ltd. (Tokyo,JP) Nippon Soda Company, Ltd. (Tokyo,JP) Nissan Chemical Industries, Ltd. (Tokyo,JP) Tosoh Corporation (Yamaguchi,JP)
A fused protein comprising a polypetide containing an antibody binding site of protein A and a polypeptide of lymphotoxin, and having biological activities of lymphotoxin and an ability to bind to an antibody is disclosed. Further, a process for the production of the fused protein, as well as a DNA coding for the fused protein, a plasmid containing the DNA, and E. coli transformed with the plasmid, necessary for the above-mentioned process, are provided.
A therapeutic agent for and the treatment of NIDDM which comprises a cytotoxic cytokine or an inducer of a cytotoxic cytokine, as the effective ingredient.
