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Description  |
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FIELD OF THE INVENTION
The present invention relates to the production of novel immunoconjugates
unstable at low pH, in particular to such immunoconjugates containing
chemotherapeutic agents and to methods of using such immunoconjugates in
chemotherapy.
BACKGROUND OF THE INVENTION
Although various chemotherapeutic drugs have been found effective against
certain tumors and even curative against some (Pardee, Devita and Hellman,
eds., in Cancer, Principles and Practice of Oncology, Lippincott & Co.
(1982)), there is a great need for therapeutic agents which kill cancer
cells more efficiently and more selectively. An attractive approach
towards meeting this need is to use antibodies to prepare antibody-drug
complexes or "immunoconjugates" that direct or "target" anti-cancer agents
to tumors. Antibodies are known in the art which recognize antigens
expressed on cancer cells, for example the antibody 96.5 which reacts with
the p97 antigen of human melanomas (Brown et al., J. Immunol., 127 p. 539
(1981)). Several immunoconjugates of this type have been shown to be
selectively cytotoxic to antigen-positive tumor cells in vitro, to
localize in tumors in vivo, and to have anti-tumor activity in mice that
is greater than that of the drug or antibody alone (Rowland et al., Cancer
Immunol. Immunotherapy, 19, pp. 1 (1985)). While the ability of such
immunoconjugates to cure human tumors remains to be demonstrated,
improvements in tumor targeting have been the focus of recent research
efforts.
For a chemotherapeutic agent to be able to exert an effect on tumors, it
must be taken up by the tumor cells, since very few, if any, cancer drugs
are otherwise cytotoxic. The immunoconjugates must, therefore, be directed
to the cancer cells, for example by antibody recognition of
tumor-associated antigens, and either be taken up by the cancer cells
(with active drug being released inside the cells), or the active drug
must be released in the close vicinity of the cancer cells, and
internalized in the same way as when the drug is used conventionally. The
second alternative has several advantages. First, while anti-cancer drugs
can be taken up by most cells, the internalization of immunoconjugates
depends on both the antigenic target of the respective antibody and the
cell in which the antigen is expressed. Antibodies to antigens that
undergo modulation, i.e., those antibodies that are internalized in the
form of an antigen-antibody complex (Old et al., Proc. Soc. Exp. Biol.
Med., 124, p. 63 (1967)), are the ones most easily used for drug targeting
(Jansen et al., Immunol. Rev., 62, p. 185 (1982)). Second, there is
heterogeneity in the expression by cells of most tumor antigens so that
cells which do not express a given antigen, i.e., are antigen-negative,
frequently occur within a tumor (Yeh et al., J. Immunol., 126, p. 1312
(1981); Albino et al., J. Exp. Med., 154, p. 1764 (1981)). Although the
difficulty of accumulating effective levels of chemotherapeutic agents
within a tumor as a result of tumor cell heterogeneity can be decreased by
combining antibodies to different antigens expressed by the same tumor
cells and forming immunoconjugates, it could be further minimized if a
therapeutic approach was developed in which the presence of some minimal
amount of cells possessing the given antigen within a tumor would be
sufficient to allow localization of effective amounts of immunoconjugates.
Third, there are some tumor antigens, mucins, for example, which are
present in larger amounts outside of the cells than at the cell membrane,
(Rittenhouse et al., Laboratory Medicine, 16, p. 556 (1985)) suggesting
the potential for targeting tumor regions.
The acidity (pH) of tumor tissues appears to be lower than that of normal
tissues. Studies conducted more than half a century ago showed that
malignant tumors metabolize carbohydrates mainly by anaerobic glycolysis,
even under aerobic conditions (Warburg et al., Biochem. Z., 152, p. 309
(1924)). The oxidation of glucose stops at the stage of glucose oxidation
to pyruvic acid, followed by reduction to lactic acid (Boxer and Devlin,
Science, 134, p. 1495 (1961)). Most of this lactic acid is either removed
or buffered by surrounding extracellular fluid, but some of it accumulates
extracellularly. This results in a lower pH within the tumor than in
normal tissues. Elevation of the blood-sugar by intravenous infusion of
glucose should accelerate anaerobic metabolism resulting in even more
lactic acid in the tumor, and this should further increase the pH
difference between tumors and normal tissues.
Following Warburg's studies, there have been several reports of lower pH in
tumors of both experimental animals, (Voegtlin et al., Nat'l. Inst. Hlth.
Bull., 164, p. 1 (1935); Kahler and Robertson, J. Nat. Cancer Inst., 3, p.
495 (1943); and human patients, Naeslund, Acta Soc. Med. Upsal., 60, p.
150 (1955); Pampus, Acta Neurochir., 11, p. 305 (1963)).
Meyer et al., in Cancer Res., 8, p. 513 (1948) reported that the pH of
malignant human tumors is lower than in normal tissues. In twelve out of
fourteen cases, where both normal and neoplastic tissues from the same
patients could be studied in vivo, there was a difference in pH which
averaged 0.49 and ranged from 0.17 to 1.15.
Ashby, (Lancet, August 6, p. 312 (1966)), found that the mean pH of
malignant tumors from nine patients was 6.8 (ranging between 6.6 and 6.9).
Raising of the blood sugar by intraveneous infusion of dextrose further
decreased the tumor pH to a mean of 6.5 (range 6.3-6.8).
Van Den Berg et al., Eur. J. Cancer Clin. Oncol., 18, p. 457 (1982), showed
that the pH of twenty-two human mammary carcinomas was 7.29 (.+-.0.05,
SEM), as compared to 7.63 (.+-.0.03, SEM) in human subcutis, and observed
similar differences in rat tumors. The differences between pH in tumors
and normal tissues were highly statistically significant, although they
were lower than those reported in the studies discussed above.
Thistlethwaite et al., Int. J. Radiation Oncology Biol. Phys., 11, p. 1647
(1985), showed, likewise, that the pH of human tumors as measured by
readings on fourteen tumors was below the physiological level with an
average of 6.81.+-.0.09 (SEM). They speculated that the reported
therapeutic effectiveness of hyperthermia depends on the lower
extracellular pH of tumors as compared to normal tissues.
Trouet et al., U.S. Pat. No. 4,376,765, describe drug compounds composed of
a protein macromolecule (carrier) linked via a peptide chain ("spacer
arm") to an amino function of a drug. The carrier facilitates endocytic
take-up by target cells so that the spacer arm may be cleaved within the
cell. Recently, attention has been directed to developing antibody drug
conjugates which release a drug within a tumor cell once the conjugate has
crossed the cell membrane and encountered acidic pH (3.5-5.5) within the
cell. U.S. Pat. No. 4,569,789 by Blattler et al., describes chemical
formation of conjugates using crosslinking structures which can link
amino-group substances such as chemotherapeutic drugs to the sulfhydryl
portion of a compound such as an antibody reactive with tumor cell surface
antigens capable of crossing the tumor cell membrane. One limitation of
such a method of forming conjugates is that the antibody must contain a
sulfhydryl group. This reduces the number of possible drug-antibody
conjugates which may be formed using such procedures.
In spite of the published evidence that tumors have lower pH than normal
tissues, and that acid-cleavable complexes may be formed between
antibodies and drugs, this evidence has not yet resulted in the
development of immunoconjugates which are composed of antibodies reactive
with tumor associated antigens and chemotherapeutic agents, and which
could be targeted to tumor tissues and are capable of selectively
releasing the chemotherapeutic agents in the presence of the lower pH of
cancer tissues for uptake by the tumor cells, but not at the pH of normal
tissue.
SUMMARY OF THE INVENTION
In the present invention, pH sensitive immunoconjugates are provided for
treating tumors in mammals by delivering a chemotherapeutic agent to tumor
tissue. The immunoconjugates comprise an antibody reactive with a
tumor-associated antigen coupled to a chemotherapeutic agent by a link
which renders the conjugate unstable at low pH. In particular, the
immunoconjugates comprise a monoclonal antibody which does not have to be
internalized by tumor cells, and the chemotherapeutic agent is a compound
such as an anthracycline compound effective in the treatment of tumors and
possessing at least one free amino residue. A species of immunoconjugate
showing particularly desirable properties for pH sensitivity in the range
of pH of human tumor tissue, is that comprised of the L6 monoclonal
antibody coupled by a poly-L-Lysine spacer to the drug Daunomycin.
BRIEF DESCRIPTION OF THE DRAWINGS
The present invention will be described in connection with the accompanying
drawings in which:
FIG. 1 is a graph depicting gel chromatographs of the antibody-Daunomycin
immunoconjugate reaction mixture;
FIG. 2 is a photograph of an electrophoretic (SDS) gel of modified and
unmodified antibody;
FIG. 3 depicts the absorption spectra of the free and conjugated forms of
Daunomycin;
FIG. 4 illustrates the effects of changes in pH on the kinetics of release
of Daunomycin conjugated to human IgG;
FIG. 5 is a graph of the toxicity to melanoma cells of various doses of
free Daunomycin as measured by 3[H] thymidine uptake by cells over time;
FIG. 6 is a graph of the toxicity to lung carcinoma cells of various doses
of Daunomycin as measured by 3[H] thymidine uptake by cells over time;
FIG. 7 is a graph of the binding of the L6 antibody to lung carcinoma
cells, and of the 96.5 antibody to melanoma cells at different pH;
FIG. 8 is a graph of the competition binding assay of the
L6-Poly-L-Lysine-Daunomycin (L6-PLS-ADM) conjugate to the fixed cell line
3347 (M285 designates the conjugate);
FIG. 9 is a graph of thymidine inhibition of the L6-PLS-ADM conjugate;
FIG. 10 is a graph of colony inhibition showing toxicity of the L6-PLS-ADM
conjugate at pH 6 and pH 7 (M214 designates the conjugate);
FIG. 11 is a graph showing the blood clearance of the L6-PLS-ADM conjugate
in nude mice. This graph compares the blood clearance of the conjugate to
native L6 antibody and to the non-specific antibody IF5;
FIG. 12 is a graph of the in vivo tumor uptake of L6-PLS-ADM conjugate, L6
antibody alone and the non-specific IF5 antibody;
FIG. 13 is a graph of the localization index (L.I.) as a function of time
for the L6-PLS-ADM conjugate and the native L6 antibody;
FIG. 14 is a graph of the in vivo kidney uptake of the L6-PLS-ADM
conjugate, L6 antibody alone and the IF5 non-specific antibody; and
FIG. 15 is a graph of the in vivo liver uptake of the L6-PLS-ADM conjugate,
L6 antibody alone and the IF5 non-specific antibody.
Accordingly, the present invention provides novel immunoconjugates composed
of antibodies selectively reactive with tumor-associated antigens to
target tumor tissues linked to chemotherapeutic agents. The
immunoconjugates are unstable in low pH tumor tissues. The conjugates have
a low toxicity at the pH of normal tissue, but when the conjugates
localize in low pH tumor tissue as a result of recognition by the
antibodies of the antigens associated with tumor cells because of the
chemical instability of the conjugates, the chemotherapeutic agent is
released and can be taken up by the tumor cells. Therefore, it is
unnecessary for the entire conjugate to be internalized within the tumor
cell, i.e., for the antibody to cross the cell membrane, for cell death to
occur. In addition, those tumor cells which lack the target antigen can
still be killed by the chemotherapeutic agent, provided a sufficient
number of cells within the tumor express the antigen recognized by the
antibody of the immunoconjugate. In addition, the invention includes
methods for using these pH-sensitive immunoconjugates in chemotherapy, by
introducing the conjugates into a patient to localize in low pH tumor
tissue, where the chemotherapeutic is released and allowed to diffuse into
the tumor cells. Thus, the expression of tumor-associated antigens in only
a minimal number of the targeted tumor cells or tumor-associated tissue is
required for tumor therapy, using the present invention. The examples set
forth below demonstrate the ability of immunoconjugates prepared according
to the invention, to localize in tumor tissue in an animal model.
To form the immunoconjugates of this invention, suitable antibodies must be
selected or developed. The antibodies used for the conjugates are
preferably monoclonal antibodies of either mouse or human origin, which
are reactive with antigens that are expressed most strongly at the surface
of tumor cells and/or in the close vicinity (i.e. outside the cell
membrane) of tumor cells. Monoclonal antibodies may be produced using
procedures such as those described by Kohler and Milstein in Nature, 256,
p. 495, (1975). An example of one such monoclonal antibody, and the
antibody preferred for use in this invention, is the L6 antibody (American
Type Culture Collection "ATCC," No. HB8677), an IgG2a mouse immunoglobulin
which is specific for a gaglioside antigen and which reacts with most
human carcinomas. The ganglioside antigen (referred to as the "L6
antigen"), is expressed at the surface of cells of most human carcinomas,
including non-small lung carcinomas, breast carcinomas, colon carcinomas
and ovarian carcinomas. The L6 antibody and the L6 antigen are described
in copending U.S. patent application Ser. No. 684,759, and
continuation-in-part application, Ser. No. 776,321 which were filed on
Dec. 21, 1984, and Oct. 18, 1985, respectively, and assigned to the same
assignee as the present invention, the disclosure of which is incorporated
by reference herein. The L6 antigen does not modulate in the presence of
L6 antibody (i.e., the antigen antibody complex is not internalized),
indicating that the L6 antibody remains at the cell surface and is not
taken up by tumor cells.
Additional monoclonal antibodies of mouse, rat, human or other origin can
be generated to the L6 antigen, or other tumor-associated antigens.
Chimeric antibodies, obtained by splicing together genes for the variable
region of the antibody molecule (of mouse origin) and genes for the
constant region (of human origin) as are exemplified by the work of
Morrison et al., Proc. Natl. Acad. Sci., 81, p. 6851 (1984), and Takeda et
al., Nature, 314, p. 452 (1985), may also be used. The immunoconjugates
can also be made by using polyclonal sera which are prepared in various
species, including rabbits and monkeys. Various fragments which are, for
example, obtained by proteolytic digestion of antibody molecules, and
include Fab, (Fab').sub.2, and Fc fragments can also be used. The present
invention can equally well be carried out by using antibodies and
fragments which are specific for antigens other than the L6 antigen, as
long as the antibodies and fragments have a high affinity constant
(10.sup.8 M or better) and the antigen is either expressed in high levels
at the tumor cell surface (at least 50,000 molecules per cell) or is
present at relatively high levels in the immediate vicinity of the tumor
cells.
Suitable chemotherapeutic agents for use in the present invention are those
which have a cytotoxic and/or growth inhibitory effect on cancer cells.
These include therapeutic agents of the type commonly used in the
treatment of human cancer, including antineoplastic drugs such as the
anthracycline compounds Daunomycin, Mitocycin C, Adriamycin, and
antimetabolites such as the folic acid antagonist, for example,
Methotrexate.
In the present invention, the immunoconjugates must be unstable at low pH
to release the chemotherapeutic agent. This may be accomplished using
several methods of chemical synthesis. In one approach, a pH-sensitive
link such as aconitic anhydride, is attached to a chemotherapeutic agent
and the carboxyl group (--COOH) of the agent is then coupled to the lysine
group of the antibody. This approach is similar to the chemistry described
by Shen and Ryser, Biochem. Biophys. Res. Comm., 102, p. 1048 (1981),
incorporated by reference herein. Stable immunoconjugates between toxins
and antibodies to certain lymphocyte populations for carrying the
conjugate into the target cells, have been developed using such
procedures; these immunotoxins have been found to be immuno-suppressive.
Diener et al., Science, 231 p. 148 (1985).
The pH unstable immunoconjugates of the present invention may also be
formed using an aconitic anhydride link to couple the chemotherapeutic
agent to the antibody. These reactions are depicted below. In Step I of
such a procedure, the labile gamma-carboxyl group of aconitic anhydride is
reacted with a suitable chemotherapeutic agent, such as Daunomycin,
containing at least one free amino group forming an intermediate compound
(1). In the next step (II), this intermediate is reacted with an available
antibody containing at least one lysine group, in the presence of
carbodiimide reagent to form an immunoconjugate consisting of Daunomycin
and antibody coupled by the link. This immunoconjugate (2) will dissociate
in low pH medium such as tumor tissue as shown in step III.
##STR1##
When the above chemistry is used to conjugate a monoclonal antibody such as
L6 to a chemotherapeutic agent, for example, the anthracycline Daunomycin,
relatively low yields of reaction may be obtained so that the amount of
drug associated with antibody, which will be released, may be too low for
optimum therapeutic effectiveness. In addition, the reactivity of the
antibody may be affected by a polymerization reaction induced by the
carbodiimide reagent used in the above reaction. Therefore, although the
reaction may be used to form the immunoconjugates of this invention, it is
preferable to improve the above reaction, for example, by using activating
reagents, or by the use of spacer molecules.
Thus, to improve the reactions, a succinated intermediate of the anhydride
modified chemotherapeutic agent and N-hydroxysuccinimide may be prepared
using a carbodiimide reagent such as 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide hydrochloride (EDC) to promote the activation of the
carboxylic groups of the aconitic anhydride. This intermediate is then
reacted with the amino group of an available lysine of the antibody to
form an immunoconjugate with an amide bond. Such immunoconjugates are
described, and the reactions shown, in Example II below.
Particularly useful immunoconjugates may be prepared which incorporate
spacer molecules, preferably polyamino acids containing at least three
amino acids such as poly-L-Lysine and poly-L-Glutamic acid and including
protein molecules, for example, albumin. In a preferred conjugation
process, the amino group of a lysine in a lysine-containing antibody is
modified by thiolation, for example using
S-acetylmercaptosuccinicanhydride (SACA) to provide free sulfhydryl groups
(--SH). A spacer molecule, such as poly-L-Lysine is complexed with the
anhydride modified chemotherapeutic agent prepared as described above, and
the lysine group of the spacer molecule of the complex is then modified
with a reagent such as maleiimide reagent for example,
sulfosuccinimidyl-4-(N-maleimidomethyl) cyclohexane-l-carboxylate. The
thiolated antibody is then conjugated with the maleiimide-modified spacer
molecule-chemotherapeutic agent complex to form an immunoconjugate capable
of dissociation at low pH.
Alternatively, in a series of reactions mediated by a reagent such as
N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP), lysine groups in a
spacer molecule such as albumin are attached to the carboxyl group of the
anhydride-modified chemotherapeutic agent (obtained as described above),
and to the amino group of a lysine in the antibody. Immunoconjugates
containing spacer molecules are set forth in Examples IV and V below.
Immunoconjugates having a spacer link may thus be prepared with several
molecules of chemotherapeutic agent per antibody molecule (up to 50
molecules of agent per antibody molecule) which, in turn, enhances drug
delivery to the tumor tissue, without significantly altering the
reactivity of the antibody.
The level of conjugation using the above-described procedures may be
further improved by modifying the pH of the reactions so that the pH is in
the range of from 6.5 to 8.5, or by increasing the temperature during the
reactions in the range of from 4.degree. C. to 37.degree. C. Additionally,
the time of incubation may be modified to increase the amount of drug
coupled to antibody from 3 up to 24 hours. Further, the ratio of
chemotherapeutic agent introduced to the antibody in Step II of the
reaction between antibody and the chemotherapeutic agent may be changed;
final ratios of agent to antibody from 10 to 50 are preferred.
For the above approach of making the low pH unstable immunoconjugates using
an aconitic anhydride link, the chemotherapeutic agent should possess at
least one free amino group. Since the amino group is believed to be
necessary for biological activity, the spacer is preferably completely the
amino group. Suitable chemotherapeutic agents which meet these
requirements are the anthracycline compounds Daunomycin, Mitomycin C,
Adriamycin, and methotrexate. These compounds also contain a quinone
structure and an acyl (--COR) moiety, both of which are believed to be
important for tumor cell destruction. A pH stable conjugate can be made,
as a control, by using another spacer, maleic anhydride, in place of the
aconitic anhydride.
A second approach for linking a chemotherapeutic agent to an antibody to
form pH unstable immunoconjugates is based on chemical reactions using
cyanogen bromide, similar to those described by Axen et al., in Nature,
214, p. 1302 (1967), incorporated by reference herein. Axen et al.
describes coupling proteins to polysaccharide resins such as Sephadex. To
carry out these reactions, the chemotherapeutic agent, for example,
Daunomycin, is activated using cyanogen bromide (CNBR) at an alkaline pH
(e.g., pH 11.0). The activated Daunomycin is then added to a solution of
an appropriate antibody and a buffer solution, such as a sodium
bicarbonate solution, to maintain an alkaline pH. The resulting conjugate
is purified, for example, by column chromatography. The immunoreactivity
of the conjugated antibody is tested by procedures such as immunohistology
using the PAP technique (Garriques et al., Inter. S. Cancer, 29 p. 511
(1982)), or by radioactive binding assays. Immunoconjugates formed in this
manner may then be tested in the pH range of tumor tissues, preferably in
the range of from pH 5.6 to pH 6.7. These reactions may be summarized as
follows. Briefly, the carbon of the cyanogen bromide is reacted with
hydroxyl (--OH) groups of the chemotherapeutic agent to form a mixture of
intermediates (1). The intermediates are reacted with the amino group of a
lysine amino acid of the antibody to form a mixture of immunoconjugates
(2), having an amino group of a lysine amino acid of the antibody coupled
via a carboxyl link to hydroxyl groups of the chemotherapeutic agent.
These reactions are:
##STR2##
Since the immunoconjugates (2) are not stable at low pH, the conjugate
dissociates in low pH medium into free drug and antibody as shown below.
##STR3##
The chemotherapeutic agent should contain at least two hydroxyl groups,
and the antibody should contain at least one lysine amino acid for these
reactions.
A third approach for forming acid-cleavable bonds uses diazotization,
following a method described by Cuatrecasas for forming conjugates, J.
Biol. Chem., 245, p. 3059 (1970), incorporated by reference herein, and
consists of the following steps. The chemotherapeutic agent, for example,
Daunomycin, is activated using a reagent such as P-nitro benzyl chloride
in solution. After incubation, the nitro group is reduced, using a
stannous chloride solution. The product of this reduction is then
diazotized by adding HCl while cooling on ice. Sodium nitrite is added to
induce diazotization. The activated Daunomycin is then conjugated with an
available tyrosine amino acid of a suitable antibody using sodium
bicarbonate to form a nitro-benzoyl link between the antibody and the
drug. The pH is then adjusted, preferably to a pH of approximately 8.0.
The conjugated antibody is then purified using column chromatography, and
the immunoreactivity of the conjugated antibody is tested. These
immunoconjugates are tested for release of the Daunomycin at pH in the
range of 5.6 to 6.7. These reactions are represented as follows:
##STR4##
Since the immunoconjugate is unstable at low pH, the drug will be released
into the tumor tissue by dissociation (Step V).
##STR5##
The chemotherapeutic agent employed in the above reactions (the third
approach), should contain at least one hydroxyl (--OH) group, and
preferably the antibody should contain at least one tyrosine amino acid in
its structure.
The following examples are presented to illustrate the present invention
and to assist one of ordinary skill in the art in making and using the
same. The examples are not intended in any way to otherwise limit the
scope of the disclosure or the protection granted by Letters Patent
hereon.
EXAMPLE I
pH of Tumor Tissue in Humans
To investigate the in vivo pH of several types of tumor tissues in humans,
the following study was performed at the Virginia Mason Hospital in
Seattle, Wash., in March of 1986.
Ten patients (8 females and 2 males; mean age 67.3 years) with different
types of tumors were entered in an acute study during surgery. A flexible
pH probe, diameter 1.2 mm (Microelectrode 20142, Microelectrodes, Inc.,
New Hampshire, U.S.A.) connected to a digital pH meter (Bechman Model
3500) was inserted into normal tissue and tumor tissue through a 14-gauge
needle with the patient's index finger connected to a reference electrode
(NMI-401, Microelectrodes, Inc.). The probe was calibrated before and
after the procedure for each patient by use of commercially available
buffers, pH 7, (Beckman) and pH 2 (Ricca Chemicals, Arlington, Tex.). The
probe was sterilized with Turgicos solution (Johnson & Johnson, Arlington,
Tex.). The pH values were recorded after stabilization, usually within
5-10 minutes in normal tissue, and the same procedure was repeated in
tumor tissue. Two of the patients received 50 ml of a 50% glucose solution
intraveneously ("i.v."). The glucose was given over a 30 min. period
beginning one hour before surgery. The findings of this study are
summarized in Table 1, which demonstrates a consistent, highly significant
difference (0.8 pH units) between various tumors and normal tissue.
TABLE 1
__________________________________________________________________________
pH Measurements in Tumors and Normal Tissues from Ten Patients
A B
Given
pH of
pH of
i.v.
normal
tumor
Difference
Age Sex
Diagnosis glucose
issue
tissue
(A - B)
__________________________________________________________________________
(1)
76 F Cancer of the
Yes 7.2 Subc.
5.9 1.3
colon with mets
(2)
57 M Undif. mesenchymal
Yes 7.4 Subc.
6.6 0.8
tumor
(3)
80 F Rectal cancer
No 6.9 Para-
6.4 0.5
rectal
(4)
46 F Mammary cancer
No 7.4 Subc.
6.7 0.7
(5)
68 F Malignant melanoma
No 6.9 Subc.
6.0 0.9
(6)
48 M Lymphoma with
No 7.4 Subc.
6.7 0.7
axillary mets
(7)
78 F Cancer of the
No 6.9 Subc.
6.0 0.9
cardia
adenocarcinoma
(8)
77 F Mammary cancer
No 7.1 Subc.
6.5 0.6
mets
(9)
76 F Hypernephroma
No 7.3 Subc.
6.6 0.7
(10)
67 F Cancer of the
No 7.3 Subc.
6.2 1.1
esophagus
__________________________________________________________________________
Mean .+-. SEM = 7.2 .+-. 0.1 normal tissue.
Mean .+-. SEM = 6.4 + 0.1 tumor tissue.
mets = metastasis
Subc. = subcutaneous
P value = 1.9.sup.-06
EXAMPLE II
Daunomycin-Antibody Immunoconjugate
Preparation of Anhydride-Modified Daunomycin (ADM)
12 mg of Daunomycin ("DM") (Sigma Chemical Co., St. Louis, Mo.) were
dissolved in ice-cold water, and a solution of 3 ml dioxane containing 12
mg of cis-aconitic anhydride was added drop-wise. The pH was adjusted to
9.0 by the addition of 0.5N NaOH. The mixture was stirred for fifteen
minutes, after which the pH was decreased to 7 by adding 0.5M HCI. The
solution ("ADM solution") was stirred for an additional hour. This
derivative was designated "ADM".
The proportion of free (unmodified) to modified DM ("ADM") was estimated
using thin-layer chromatography on a mixture of acetone:chloroform:acetic
acid (17:3:1). The "Rf" of free drug was approximately 0.1 and that of the
spacer-DM (hereafter ADM) was approximately 0.5. Spectroscopy showed that
both DM and ADM had absorbance peaks at 475 nm and at 280 nm. (FIG. 3).
Preparation of Antibody-Daunomycin Immunoconjugate
L6 antibody (ATCC No. HB8677), was dissolved in phosphate buffered saline
(PBS), pH 7, and 0.6 ml of the ADM solution prepared as described above
was added drop-wise to 10 mg of the L6 antibody in 0.8 ml of PBS.
Subsequently, 10 mg of (1-ethyl-3)3-dimethylamino-propyl) carbodiimide
hydrochloride (EDC) was added, and the mixture was kept at 4.degree. C.
and at a pH of 7.0 for 3 hours. The mixture was then loaded onto a
Sephadex G-50 column (38.times.1.8 cm), and 1 ml fractions were collected.
The antibody-drug conjugate exhibiting the yellow color of the drug, was
eluted in fractions 16 and 17, and the free DM was eluted in fractions
35-42, as shown in FIG. 1. The yield of the conjugation reaction was
7-10%, and a ratio of 3:1 DM molecules per antibody molecule was obtained.
Tests by immunohistology, following the PAP procedures of Garrigues et al.,
supra, incorporated by reference herein, were performed to study the
ability of the conjugate to bind to tumors expressing the L6 antigen. The
tests showed that the immunoreactivity of the conjugate was preserved,
although it was weaker than that of the native antibody. These tests were
followed by cell binding assays using techniques described by Beaumier et
al., J. Nuclear Med., 27 p. 824 (1986). Approximately 80% of the original
immunoreactivity was preserved. Gel-electrophersis (7% SDS) showed only
one band of the conjugated protein. This band was identical to that of
unmodified IgG (MW, 150k), FIG. 2, indicating that most of the conjugate
remained in a monomeric state and did not polymerize.
An absorption spectrum of the purified product showed a new peak at 370 nm.
(FIG. 3). This peak indicates that a convalent bond was formed in the
conjugation between the DM and the L6 antibody.
Release of Daunomycin From the Immunoconjugate at Low pH in a Cell-Free
Medium
The purified immunoconjugate was mixed with citrate-phosphate buffers of
four different pHs: pH 4, 5, 6 or 7, after which the mixtures were
incubated at 37.degree. C. and 1 ml aliquots removed at different time
intervals. In order to separate DM which was released from the conjugate,
conjugates were filtered through a Centricon-10 Filter (Amicon, Danvers,
Mass.) which has a filtration cut-off at 10,000 daltons molecular weight,
after which the absorbance of the supernatant was checked for presence of
free DM (which absorbs at 475 nm). FIG. 4 depicts data obtained with a
conjugate prepared by coupling DM to human IgG which serves as a readily
available model for conjugation, rather than to the L6 antibody. FIG. 4
shows that after 24 hours of incubation at pH 4 or 5, between 30-40% of
the DM has been released from the conjugate. At pH 6 approximately 15% of
the DM was released. No significant release was noticed at a neutral pH.
Cytotoxicity of Daunomycin on Cultured Cell Lines
The ability of DM to inhibit .sup.3 [H] thymidine uptake by cells from an
explanated human lung carcinoma, 2981 (Oncogen, Seattle, Wash.), which can
bind the L6 antibody, and by cells from melanoma M-2669 (Oncogen, Seattle,
Wash.), which cannot, was measured. As shown in FIG. 5, free DM was very
effective even at a low dose, less than 0.5 .mu.g/ml. Cytotoxicity was
observed after only 16 hours incubation with the drug, as illustrated in
FIG. 6.
Binding of Antibody to Tumor Cells
The antibody used to form the immunoconjugate herein, L6, as well as
another antibody 96.5, demonstrate the ability to bind to tumor cells
(lung carcinoma and melanoma) in the range of pH from 5 to 7. (FIG. 7).
Thus, antibody binding is not likely to be inhibited by the pH found in
tumor tissue. (Table 1).
EXAMPLE III
Amide-Linked Daunomycin-Antibody Immunoconjugate
Preparation of Succinated ADM
To maximize the amount of chemotherapeutic agent associated with the
antibody of the immunoconjugates of this invention, ADM solution was
prepared as described above in Example II. To 4 ml of ADM solution, 10 mg
of N-hydroxysuccinimide (Fluka, Basel, Switzerland) and 5 mg of EDC was
added. This mixture was stirred at room temperature for 24 hours (pH 5) to
make the succinated product ("ADM-SUC").
Conjugation to Antibody
1.0 ml of ADM-SUC was added to 1 ml of L6 antibody (5 mg/ml in PBS buffer).
The pH was adjusted to 8.5 with 1M NaOH. The mixture was incubated for 24
hours at 4.degree. C., then purified using a G-50 sephadex column. The
immunoconjugate was isolated as described in Example II, and contains an
amide link between the antibody and the Daunomycin. The reactions may be
depicted as follows:
##STR6##
The conjugation yield was higher for the reactions in this example, and a
DM to antibody ratio of 10:1 was obtained.
EXAMPLE IV
Daunomycin-Antibody Immunoconjugate Using Albumin Spacer
Modification of Antibody
To 1-ml of the antibody L6 (5 mg/ml) was added 63 .mu.l of a solution of
SPDP (7 mg/5 ml ethanol), and the mixture was incubated for 30 minutes at
room temperature to modify a lysine amino acid of the antibody. The
SPDP-modified antibody was then purified on a PD-10 (Pharmacia, Sweden)
chromatography column, prewashed with a 0.1M sodium acetate solution (pH
4.5). The eluted peak was then reduced with 0.24 ml of dithiothreitol
(DTT) (0.5M) for 10 minutes.
Attachment of Albumin to Daunomycin
1 ml of human serum albumin (HSA) was added to 0.65 ml of
anhydride-modified Daunomycin (ADM) solution (prepared as described in
Example II). 20 mg of EDC were added to the mixture to form a DM-HSA
complex and incubated for 20 hours at 4.degree. C. The complexed ADM-HSA
was then purified on a G-50 sephadex column. The molar ratio of ADM to HSA
was 7:1.
Modification of ADM-HSA
The ADM-HSA solution was incubated with 21 .mu.l of SPDP solution (7 mg/5
ml of ethanol) for 30 minutes at room temperature to form SPDP modified
(ADM-HSA) which was then purified on a PD-10 column.
Conjugation
The reduced, SPDP modified L6 antibody and the SPDP modified ADM-HSA were
then mixed together to form an immunoconjugate of Daunomycin coupled to
albumin by an albumin spacer. The ratio of DM to albumin was approximately
7:1. The reactions were:
##STR7##
EXAMPLE V
Daunomycin-Antibody Immunoconjugate Using Poly-L-Lysine Spacer
Preparation of Anhydride-Modified Daunomycin (ADM)
Sixteen (16) mg of Daunomycin ("DM") (Sigma Chemical Co., St. Louis, Mo.)
were dissolved in 1.5 ml of ice-cold water. 16 mg cis-aconitic anhydride
was slowly added to the dissolved Daunomycin. The pH was adjusted to 9.0
by the addition of 0.5N NaOH. The mixture was stirred for 15 min, and the
pH was then decreased to 3 by adding HCl. The solution was stirred in the
cold (4.degree. C.) for 15 min. The pellet was then isolated by
centrifugation for 15 min at 4.degree. C. at 3000 rpm. The pellet was
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