Two Bacillus species and one Pseudomonas species isolated from soil samples showed good cyclodextrin glycosyltransferase activity, even when grown in simple media. The microorganisms are not pH sensitive and grow well in media containing only starch, a nitrogen source, and minerals. All elaborate the desired enzyme within 4 hours of culturing with growth being complete within about 24 hours.
The present invention relates to the preparation of a cylodextrinase having specific physicochemical characteristics relating to action, substrate specificities, optimum pH, stable pH, inhibition, activation and molecular weight by culturing a specified microorganism belonging to the genus Bacillus, and also provides a process for preparing maltooligosaccharides by using the present enzyme on cylodextrins.
The present invention relates to a novel cyclodextrinase having the specific physicochemical characteristics relating to action, substrate specificities, optimum pH, stable pH, inhibition, activation and molecular, weight. This novel enzyme can be produced by culturing a specified microorganism belonging to the genus Bacillus. The invention also provides a process for preparing maltooligosaccharides by acting the present enzyme to cyclodextrins.
