The invention relates to Aureobasidium pullulans strains which produce pullulan but virtually no melanin. A process for the preparation of these strains, and a preferred use, are also disclosed.
CROSS REFERENCE TO RELATED APPLICATION
This is a continuation application of prior copending U.S. Pat. application Ser. No. 925,708, filed Oct. 30, 1986, and now abandoned.
A method for obtaining a substantially biologically pure culture strain of Aureobasidium pullulans from a wild type strain by enriching the collected strain for organisms which grow as fungal yeastlike cells, growing colonies from isolated yeastlike cells and selecting those yeastlike cells which exhibit reduced pigmentation. Biologically pure culture strains obtained by the invention as well as methods for producing pullulan having decreased pigmentation and/or increased molecular weight are disclosed.
A .beta.-glucan-containing fat and oil composition contains .beta.-glucan of microorganism origin or basidiomycete origin. The .beta.-glucan-containing fat and oil composition has .beta.-glucan uniformly dispersed in a food without worsening the texture, taste etc. of the food. The novel microorganism can efficiently produce .beta.-glucan which has a high activity and favorable qualities as .beta.-glucan of microorganism origin as described above from less expensive saccharides such as sucrose at a high production speed.
An antibiotic R106 represented by the general formula I): ##STR1## wherein: R is methyl or ethyl; X.sub.1 is MePhe, .beta.-HOMePhe or Phe; X.sub.2 is allo-Ile, Val or Leu; X.sub.3 is MeVal or Val; X.sub.4 is .beta.-HOMeVal, .gamma.-HOMeVal, MeVal, Val, N,.beta.-MeAsp, .beta.-HOMePhe, MePhe, MeDH.sub.2,3 Val or MeDH.sub.3,4 Val is produced by a process which comprises culturing a strain of the genus Aureobasidium that is capable of producing the said antibiotic R106 and collecting the said antibiotic from the fermentation broth. The antibiotic R106 compounds are useful in the treatment of fungal infection.
An antibiotic R106 represented by the general formula (I): ##STR1## wherein: R is methyl or ethyl; X.sub.1 is MePhe, .beta.-HOMePhe or Phe; X.sub.2 is allo-Ile, Val or Leu; X.sub.3 is MeVal or Val; X.sub.4 is .beta.-HOMeVal, .gamma.HOMeVal, MeVal, Val, N,.beta.-MeAsp, .beta.-HOMePhe, MePhe, MeDH.sub.2,3 Val or MeDH.sub.3,4 Val is produced by a process which comprises culturing a strain of the genus Aureobasidium that is capable of producing the said antibiotic R106 and collecting the said antibiotic from the fermentation broth. The antibiotic R106 compounds are useful in the treatment of fungal infection.
