Immunoassay including lyophilized reactant mixture - Patent Review 5102788

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Immunoassay including lyophilized reactant mixture

United States Patent

5102788

Link to this page

http://www.wikipatents.com/5102788.html

Inventor(s)

Cole; Francis X. (Stow, MA)

Abstract

A lyophilized mixture of reactants for an immunoassay includes antibody-gold sol particle conjugates, antibody latex particle conjugates, polyethylene glycol, a polyethylene glycol p-isooctylphenyl ether detergent and a sugar such as dextrin or trehalose. The polyethylene glycol is present to enhance binding of the immunoreactants and the polyethylene glycol p-isooctylphenyl ether detergent is present to prevent non-specific interactions. The sugar prevents agglomeration of the polyethylene glycol and polyethylene glycol p-isooctylphenyl ether in the lyophilized mixture at room temperature and facilitates retention of a homogenous distribution of the ingredients of the mixture to thereby enhance shelf life and redistribution of the mixture in an aqueous test system.

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U.S. References

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3825410	Your vote accepted [0 after 0 votes]		3880715	Your vote accepted [0 after 0 votes]
4931385 Block 435/7.94 Jun,1990	Your vote accepted [0 after 0 votes]		4859612 Cole 436/523 Aug,1989	Your vote accepted [0 after 0 votes]
4853335 Olsen 436/523 Aug,1989	Your vote accepted [0 after 0 votes]		4681782 Ozkan 428/35.7 Jul,1987	Your vote accepted [0 after 0 votes]
4659666 May 435/188 Apr,1987	Your vote accepted [0 after 0 votes]		4615972 Gallacher 435/28 Oct,1986	Your vote accepted [0 after 0 votes]
4545452 Moring 180/378 Oct,1985	Your vote accepted [0 after 0 votes]		4503143 Gerber 435/7.36 Mar,1985	Your vote accepted [0 after 0 votes]
4459359 Neurath 435/5 Jul,1984	Your vote accepted [0 after 0 votes]		4459358 Berke 436/170 Jul,1984	Your vote accepted [0 after 0 votes]
4458014 Ebersole 435/7.32 Jul,1984	Your vote accepted [0 after 0 votes]		4457916 Hayashi 424/85.2 Jul,1984	Your vote accepted [0 after 0 votes]
4448882 Brodbeck 435/188 May,1984	Your vote accepted [0 after 0 votes]		4446232 Liotta 435/7.93 May,1984	Your vote accepted [0 after 0 votes]
4444879 Foster 435/7.95 Apr,1984	Your vote accepted [0 after 0 votes]		4386224 Deetman 568/703 May,1983	Your vote accepted [0 after 0 votes]
4385114 Guthlein 435/28 May,1983	Your vote accepted [0 after 0 votes]		4343896 Wolters 435/5 Aug,1982	Your vote accepted [0 after 0 votes]
4340395 Magers 436/66 Jul,1982	Your vote accepted [0 after 0 votes]		4310504 Derfler 436/500 Jan,1982	Your vote accepted [0 after 0 votes]
4299916 Litman 435/6 Nov,1981	Your vote accepted [0 after 0 votes]		4287300 Gibbons 435/5 Sep,1981	Your vote accepted [0 after 0 votes]
4280816 Elahi 436/518 Jul,1981	Your vote accepted [0 after 0 votes]		4273868 Walter 435/14 Jun,1981	Your vote accepted [0 after 0 votes]
4256833 Ali 435/7.95 Mar,1981	Your vote accepted [0 after 0 votes]		4248965 Mochida 435/7.92 Feb,1981	Your vote accepted [0 after 0 votes]
4230664 Cais 422/61 Oct,1980	Your vote accepted [0 after 0 votes]		4206200 Guthohrlein 424/250.1 Jun,1980	Your vote accepted [0 after 0 votes]
4200690 Root 435/7.22 Apr,1980	Your vote accepted [0 after 0 votes]		4188371 Weetall 435/7.36 Feb,1980	Your vote accepted [0 after 0 votes]
4169012 Dawson 435/7.9 Sep,1979	Your vote accepted [0 after 0 votes]		4157280 Halbert 435/5 Jun,1979	Your vote accepted [0 after 0 votes]
4115535 Giaever 436/526 Sep,1978	Your vote accepted [0 after 0 votes]		4108974 Wegfahrt 436/500 Aug,1978	Your vote accepted [0 after 0 votes]
4094647 Deutsch Jun,1978	Your vote accepted [0 after 0 votes]		4062933 Wolfangel 424/1.37 Dec,1977	Your vote accepted [0 after 0 votes]
4017597 Reynolds 435/7.92 Apr,1977	Your vote accepted [0 after 0 votes]		3987159 Spona 436/521 Oct,1976	Your vote accepted [0 after 0 votes]
3963441 Dietrich 435/7.22 Jun,1976	Your vote accepted [0 after 0 votes]		3791932 Schuurs 435/7.8 Feb,1974	Your vote accepted [0 after 0 votes]
4486530 David 435/7.91 Dec,1969	Your vote accepted [0 after 0 votes]		4391904 Litman 435/7.91 Dec,1969	Your vote accepted [0 after 0 votes]
4228240 Dawson 435/188 Dec,1969	Your vote accepted [0 after 0 votes]
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Claims

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We claim:

1. A lyophilized mixture for use in an immunoassay procedure comprising:

at least one dispersible immunoreactive component distributed homogeneously throughout said mixture;

at least one organic component distributed homogeneously throughout said mixture, said organic component normally being a liquid at the conditions under which the mixture is stored or retained prior to use and having a property which enhances the performance of the immunoassay by its presence; and

a sugar comprising dextrin or trehalose, said sugar being present in said mixture in sufficient quantity to prevent agglomeration of the organic component and thus maintain the homogeneity of the mixture to thereby facilitate storage and shelf life of the mixture and the eventual dispersion of the mixture in an aqueous medium for conduct of the immunoassay procedure.

2. A lyophilized mixture as set forth in claim 1, wherein said immunoreactive component comprises an antibody conjugate.

3. A lyophilized mixture as set forth in claim 2, wherein said antibody conjugate comprises an antibody-enzyme conjugate.

4. A lyophilized mixture as set forth in claim 1, wherein said organic component comprises a binding enhancing agent.

5. A lyophilized mixture as set forth in claim 4, wherein said binding enhancing agent comprises a non-ionic, water soluble polymer.

6. A lyophilized mixture as set forth in claim 5, wherein said polymer comprises polyethylene glycol.

7. A lyophilized mixture as set forth in claim 5, wherein said polymer comprises polyvinyl alcohol.

8. A lyophilized mixture as set forth in claim 5, wherein said polymer comprises polyvinyl pyrrolidone.

9. A lyophilized mixture as set forth in claim 5, wherein said polymer comprises dextran.

10. A lyophilized mixture as set forth in claim 1, wherein, said organic component comprises a surfactant.

11. A lyophilized mixture as set forth in claim 10, wherein said surfactant comprises a water soluble non-ionic surface active agent.

12. A lyophilized mixture as set forth in claim 11, wherein said surface active agent comprises a polyethylene glycol p-isooctylphenyl ether compound.

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