Carbonyl derivatives of acetaminophen are provided for use in homogeneous enzyme immunoassays for acetaminophen. The derivatives are conjugated to antigenic substances for the preparation of antisera specific to acetaminophen, and to enzymes for the preparation of enzyme conjugates which compete with acetaminophen for antibody binding sites in a typical assay.
This application is a continuation of Ser. No. 06/891447, filed July 29, 1986, now abandoned, which is a divisional of Ser. No. 06/692,791, filed Jan. 17, 1985, now U.S. Pat. No. 4,605,754, which is a divisional of Ser. No. 06/552955, filed Nov. 17, 1983, now U.S. Pat. No. 4,504,413, which is a divisional of Ser. No. 06/364836filed Apr. 2, 1982, now U.S. Pat. No. 4,424,150.
Bidentate reagents for rapidly and quantitatively assaying the concentration of pharmacological agents in biological samples are described. The reagents are used in an immunoassay format for determining the concentration of desired, preselected pharmacological agents, such as benzoylecgonine, cocaine, an opiate, PCP, digoxigenin, acetaminophen, carbamazepine, phenytoin, primidone, theophylline, an aminoglycoside antibiotic, vancomycin, quinidine or a cannabinoid.
