An extract for the treatment of calcium oxalate kidney stone disease which can be extracted and purified from leaves of the plant Eriobotrya japonica is disclosed. The extract has the following characteristics: inhibits calcium oxalate crystal growth in vitro, inhibits renal calcium oxalate crystal deposition in rats which are fed ethylene glycol, causes rats which are fed ethylene glycol and said extract to excrete less oxalate in their urine as compared to rats which are fed ethylene glycol without said extract, shows little or no loss in activity when exposed to an aqueous solution of pH 1.5 for 14 hours, shows increased activity when exposed to an aqueous solution of pH 12.7 for 15 hours, is polyanionic, is stable when heated in an aqueous solution (at pH of extract) at 98.degree. C. for 3 hours, water-soluble, insoluble in heptane, hexane, chloroform/methanol mix, diethyl ether, and ethanol/aqueous mix, binds to DEAE-A-25 Sephadex at pH 8.6 and is eluted from the DEAE-A-25 Sephadex with 2 M NaCl. The present invention is also directed to a method for the treatment of calcium oxalate stone disease wherein the extract is administered, preferably for a prolonged period of time, to a patient suffering from or prone to calcium oxalate stone disease.
Dicitrate cyclic diester (dicitrate) is a novel compound which is found in patients who do not exhibit symptoms or predisposition to idiopathic renal calculous disease. Dicitrate can be isolated and detected by novel chromatographic methods. Further, treating patients prophylactically and therapeutically with dicitrate cyclic diester can prevent or reduce the effects of calcification-related disease, including some types of kidney stones, or other disease or condition associated with the presence of metal ions. The metal ion complexing properties exhibited by dicitrate are also applicable to the use of the subject compounds as chelating agents and methods of binding, and thereby reducing, the amounts of metal ions in a substance.
Treating patients prophylactically and therapeutically with dicitrate cyclic diester (dicitrate) can prevent or reduce the effects of calcification-related disease, including some types of kidney stones.
Dicitrate cyclic diester (dicitrate) is a novel compound which is found in patients who do not exhibit symptoms or predisposition to idiopathic renal calculous disease. Kits are provided for the detection of dicitrate cyclic diester.
Dicitrate cyclic diester (dicitrate) is a novel compound which is found in patients who do not exhibit symptoms or predisposition to idiopathic renal calculous disease. Dicitrate can be isolated and detected by novel chromatographic methods. Further, treating patients prophylactically and therapeutically with dicitrate cyclic diester can prevent or reduce the effects of calcification-related disease, including some types of kidney stones.
