Compounds and methods for detecting the incorporation of a hapten into a protein or macromolecules are disclosed. The compounds comprise haptens bound directly or indirectly to a chromophoric group and a macromolecules-reactive group, whereby incorporation of the hapten into a protein or other macromolecules may be detected. The disclosure includes a compound of the formula: ##STR1## wherein n.sub.1 is 1, 2, 3, 4, 5, 6 or 7; n.sub.2 is 0, 1, 2, 3, 4, 5, 6 or 7; R.sub.1 is either a carboxytetramethyl-rhodamyl, sulforhodamyl 101, or dinitrophenylaminohexanoyl group; and R.sub.2 is --CO.sub.2 H, --NH.sub.2, --SH, ##STR2##
A unique class of chemiluminescent labels containing biotin substitution that are suitable for chemiluminescent assays using inter alia a streptavidin and/or avidin conjugate. The chemiluminescent labels of the invention have the ability to bind to streptavidin and/or avidin per se or to streptavidin and/or avidin conjugated with an analyte. Label structures are disclosed that have hydrolytic stability to meet the most demanding commercial assay conditions. The invention encompasses conjugates containing associated versions of the labeling compounds, assays and kits for performing such assay utilizing the conjugates.
A method is provided for continuously monitoring for the presence or quantity of an analyte in a flowing liquid stream. The method involves binding an analyte-specific receptor species to the surface of a piezoelectric substrate, contacting the surface bound receptor species with the flowing liquid stream and quantitating the presence of the analyte. A novel apparatus for detecting the presence of an analyte in a liquid chromatography eluant is provided as well.
A nucleotide probe complex which enhances the ability to discriminate low level samples in electrochemiluminescent assays. The complex is composed of a platform molecule to which multiple copies of an organometallic electrochemiluminescent label and an oligonucleotide probe are separately attached. Preferably the complex is capped with streptavidin. Use of the complex permits detection of 1000 copies of analyte per sample in less than one hour.
A nucleotide probe complex which enhances the ability to discriminate low level samples in electrochemiluminescent assays. The complex is composed of a platform molecule to which multiple copies of an organometallic electrochemiluminescent label and an oligonucleotide probe are separately attached. Preferably the complex is capped with streptavidin. Use of the complex permits detection of 1000 copies of analyte per sample in less than one hour.
The present invention discloses novel immunogens, antibodies prepared from such immunogens, and labeled reagents useful in immunoassays for the detection and quantification of testosterone in a test sample. Also disclosed are immunoassays using these reagents and methods for synthesizing these reagents. The immunoassays are preferably microparticle enzyme immunoassays (MEIAs) and fluorescence polarization immunoassays (FPIAs). Further disclosed are novel starting materials for making the above novel immunogens and labeled reagents. Methods for making the novel immunogens and labeled reagents from the novel starting materials are also disclosed.
