The elastic-port immersion cell (10) is an optical oil immersion vessel which has a flexible latex (or other) sheath (24) protruding from its sidewall. The sheath is affixed to an opening in the wall of the vessel using a collar, O-ring (26) or other connecting means. The base of the vessel is an optical glass window. A sample (20) mounted on to the end of spindle (14) is placed into the cell. The end of the spindle (14), without the sample, is inserted into the sheath and the latex together with the spindle is attached to a goniometer head of a spindle stage such that the spindle is horizontal. Immersion oil (22) that has a refractive index similar to the sample is added to the cell until the sample is totally submerged. Minute features within the sample (20) may then be observed under the microscope as the sample is rotated 360 degrees about a vertical or horizontal axis. The latex sheath prevents the oil from escaping from the cell and also provides the flexibility required to orient and rotate a sample.
A rotary stage (10) for use in optical projection tomography includes a stepper motor (42) with a rotatable vertical shaft (44) the lower end of which carries a specimen (28) to be imaged so that the specimen is rotated about a substantially vertical axis. The stepper motor (42) is mounted on a table (34) the position of which is accurately adjustable in tilt and in vertical position to ensure that the rotational axis of the specimen is perpendicular to the optical axis (29). The specimen (28) rotates within a stationary chamber (26) and the rotary stage is used with a microscope which provides a three-dimensional image of the specimen.
A device for holding and positioning a sample (22) during spectral analysis, having an optical cell (41) comprised of a base member (42), a glass cell (44), and being filled with oil (46) having substantially the same index of refraction as the glass cell (44). The device further includes a rotary stage device (48) attached to the optical cell (41) and a sample holder (50) attached to the rotary stage device (48) such that a sample (22) may be rotated over a range of angles relative to a projected beam (32) to provide a spectral analysis of the sample (22) over the range of angles.
A system constructs a high resolution image from a plurality of low resolution images where each of the low resolution images includes a plurality of color planes. The system receives the plurality of low resolution images where the plurality of color planes of each of the low resolution images are spatially arranged in at least a partially non-coincident manner. Motion data representative of motion of at least a portion of the low resolution images is determined. The high resolution image is constructed, having a resolution greater than the low resolution images, based upon the non-coincident color planes of the low resolution images, together with the motion data.