A method for the regeneration of a cotton plant from somatic cells. The method comprises providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until the secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant and culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the callus.
CROSS-REFERENCE TO RELATED APPLICATION
This is a continuation of application Ser. No. 08/122,351, filed Sep. 14, 1993, now abandoned which is a divisional of Ser. No. 07/680,048 filed Mar. 29, 1991, now U.S. Pat. No. 5,244,802, which was a continuation of Ser. No. 07/122,200 filed Nov. 18, 1987, now abandoned, both of which are incorporated herein by reference.
This invention relates to improved methods for the production of transgenic cotton plants, comprising cocultivating Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border with cotton embryogenic callus in the presence of a plant phenolic compound.
The invention features a method of producing a grape somatic embryo having resistance to a plant pathogen, the method including the steps of (a) culturing a grape somatic embryo in a first liquid culture medium that includes a plant growth regulator and a phytotoxin from a plant pathogen; (b) exchanging the first liquid culture medium for a second liquid culture medium not including the phytotoxin; (c) recovering a living grape cell or grape cell cluster from the second liquid culture, the living cell or cell cluster being resistant to the pathogen; and (d) culturing the grape cell or grape cell cluster in a third culture medium to produce a grape somatic embryo.
The invention features a method of selecting for a grape somatic embryo or grape plant having resistance to a plant pathogen using a liquid culture medium. The method including the steps of (a) culturing a grape embryonic culture in a first liquid culture medium to grow a cellular suspension culture, the first liquid culture medium including a plant growth regulator and a filtrate from a plant pathogen culture; (b) recovering an embryogenic cell or embryogenic cell mass from the cellular suspension culture; and (c) culturing the embryogenic cell or embryogenic cell mass in a second liquid culture and in a third culture media to select a grape somatic embryo or a grape plant having resistance to a plant pathogen.
A cotton cultivar designated HAMMER is disclosed. The invention relates to the seeds of cotton cultivar HAMMER, to the plants and plant parts of cotton HAMMER and to methods for producing a cotton plant produced by crossing the cultivar HAMMER with itself or another cotton variety. The invention further relates to hybrid cotton seeds and plants produced by crossing the cultivar HAMMER with itself or with another cotton cultivar.
A cotton cultivar designated Acala ULTIMA EF is disclosed. The invention relates to the seeds of cotton cultivar Acala ULTIMA EF, to the plants and plant parts of cotton Acala ULTIMA EF and to methods for producing a cotton plant produced by crossing the cultivar Acala ULTIMA EF with itself or another cotton variety. The invention further relates to hybrid cotton seeds and plants produced by crossing the cultivar Acala ULTIMA EF with itself or with another cotton cultivar.
