A method for analyzing chemical species and determining the molecular weight of a parent molecule. Multiply charged ions are produced from the parent molecule by adding adduct ions thereto. A mass analysis is conducted of the multiply charged ions to generate mass/charge data. The molecular weight is calculated by using a deconvolution procedure in which the adduct ion mass and the molecular weight of the parent molecule are both treated as unknowns. Alternatively, a modified deconvolution procedure in which the adduct ion mass is treated as a known value is used.
RELATED APPLICATIONS
The present application is a continuation of U.S. patent application Ser. No. 08/220,369, filed Mar. 30, 1994 and issued as U.S. Pat. No. 5,440,119 on Aug. 8, 1995, which is a continuation-in-part of U.S. patent application Ser. No. 07/892,113 filed Jun. 2, 1992 and issued as U.S. Pat. No. 5,300,771 on Apr. 5, 1994.
Fully automated modular analytical systems with integrated instrumentation for analysis of biopolymer samples, such as nucleic acids, proteins, peptides and carbohydrates, are provided. Analytical methods of detection and analysis, such as mass spectrometry, radiolabeling, mass tags, chemical tags and fluorescence chemiluminescence, are integrated with robotic technology and automated chemical reaction systems to provide a high-throughput, accurate Automated Process Line (APL).
A method for detecting methylated nucleotides within a nucleic acid sample is provided. The method includes the steps of splitting a nucleic acid sample into separate reaction vessels; contacting nucleic acid in one reaction vessel with bisulfite; amplifying the nucleic acid in each reaction vessel; cleaving the nucleic acids in each reaction vessel to produce fragments thereof; and obtaining a mass spectrum of the resulting fragments from one reaction vessel and another mass spectrum of the resulting fragments from another reaction vessel.
Methods and compositions for identifying an unknown phenotype of a tissue that correlates with changes in the methylation state of the tissue comprising, nucleic acid sample from the tissue with a reagent that modifies unmethylated cytosine to produce uracil, amplifying the nucleic acid target gene region using at least one primer that hybridizes to a strand of said nucleic acid target gene region to produce amplified nucleic acids, determining the characteristic methylation state of the nucleic acid target gene region by base specific cleavage and identification of methylation sites and comparing the ratio of methylated cytosine to unmethylated cytosine for each methylation site of the nucleic acid target gene region to the ratio of methylated cytosine to unmethylated cytosine for each methylation site of a tissue nucleic acid sample of the same type having a known phenotype thereby identifying the unknown phenotype.
Methods and kits that use nucleotide analogs to confer increased accuracy and improved resolution in the analysis and sequencing of oligonucleotide mixtures are provided.
A-kinase anchor protein (AKAPs) muteins, peptides thereof, and nucleic acids encoding the peptides are provided herein. Also provided are transgenic animals, cells comprising transgenes and various methods employing such peptides.
