Hybrid molecules having translocation region and cell-binding region

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United States Patent

6022950

Link to this page

http://www.wikipatents.com/6022950.html

Inventor(s)

Murphy; John R. (Boston, MA)

Abstract

Disclosed is a hybrid molecule comprising a first part, a second part, and a third part connected by covalent bonds, (a) wherein said first part comprises a portion of the binding domain of a cell-binding polypeptide ligand effective to cause said hybrid protein to bind to a cell of an animal; (b) wherein said second part comprises a portion of a translocation domain of naturally occurring protein which translocates said third part across the cytoplasmic membrane into the cytosol of the cell; and (c) wherein said third part comprises a chemical entity to be introduced into the cell, wherein each of said first part and said third part is non-native with respect to said naturally occurring protein, and further wherein said covalent bond connecting said second part and said third part is a cleavable bond, provided that when said second part comprises a portion of a translocation domain of Pseudomonas exotoxin, said third part is not a polypeptide.

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4675382 Murphy Jun,1987	Your vote accepted [0 after 0 votes]		4666837 Harford et al. May,1987	Your vote accepted [0 after 0 votes]
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