Methods and compositions for enhancing sensitivity in the analysis of biological-based assays

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United States Patent

6027890

Link to this page

http://www.wikipatents.com/6027890.html

Inventor(s)

Ness; Jeffrey Van (Seattle, WA), Tabone; John C. (Bothell, WA), Howbert; J. Jeffry (Bellevue, WA), Mulligan; John T. (Seattle, WA)

Abstract

Methods are provided for detecting the binding of a first member to a second member of a ligand pair, comprising the steps of (a) combining a set of first tagged members with a biological sample which may contain one or more second members, under conditions, and for a time sufficient to permit binding of a first member to a second member, wherein said tag is correlative with a particular first member and detectable by non-fluorescent spectrometry, or potentiometry, (b) separating bound first and second members from unbound members, (c) cleaving the tag from the tagged first member, and (d) detecting the tag by non-fluorescent spectrometry, or potentiometry, and therefrom detecting the binding of the first member to the second member.

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539 343 A1Apr., 1993EP	Your vote accepted [0 after 0 votes]		639 647 A2Feb., 1995EP	Your vote accepted [0 after 0 votes]
711 362 B1May., 1996EP	Your vote accepted [0 after 0 votes]		6-289018Oct., 1994JP	Your vote accepted [0 after 0 votes]
WO 89/12694Dec., 1989WO	Your vote accepted [0 after 0 votes]		WO 90/13666Nov., 1990WO	Your vote accepted [0 after 0 votes]
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