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System and methods for nucleic acid sequencing of single molecules by polymerase synthesis    

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United States Patent6255083   
Link to this pagehttp://www.wikipatents.com/6255083.html
Inventor(s)Williams; John G. K. (Lincoln, NE)
AbstractThis invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created.
   














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Inventor     Williams; John G. K. (Lincoln, NE)
Owner/Assignee    
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Publication Date     July 3, 2001
Application Number     09/460,303
PAIR File History     Application Data   Transaction History
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Litigation
Filing Date     December 13, 1999
US Classification     435/91.1 435/6 435/91.2 435/91.51 436/94 536/23.1 536/24.3 536/24.33
Int'l Classification    
Examiner     Whisenant; Ethan
Assistant Examiner     Lu; Frank
Attorney/Law Firm     Townsend and Townsend and Crew, LLP
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Parent Case     CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority to U.S. Provisional Patent Application Ser. No. 60/112,078, filed Dec. 14, 1998, and Ser. No. 60/115,496, filed Jan. 11, 1999, the disclosures of which are hereby incorporated by reference in their entirely for all purposes.
Priority Data    
USPTO Field of Search     435/6 435/91.1 435/91.2 435/183 435/283.1 435/287.1 435/287.2 436/94 536/23.1 536/24.3 536/24.33 536/25.3 702/19 702/20
Patent Tags     methods nucleic acid sequencing single molecules by polymerase synthesis
   
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5952174
Nikiforov et al.

Sep,1999

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