Histone deacetylases and nucleotide sequences encoding said histone deacetylases are provided. The sequences as well as corresponding antisense constructs are useful for modulating gene activity in plants. Additionally, the sequences are useful for enhancing disease resistance in transformed plants.
This invention relates to an isolated nucleic acid fragment encoding a chromatin associated protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the chromatin associated protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the chromatin associated protein in a transformed host cell.
The present invention relates to proteins, OsHDAC1, OsHDAC2 and OsHDAC3, which function as histone deacetylase, a gene coding for said proteins, and a method for producing a plant having a high growth rate by expressing said gene in the plant. According to the present invention, the OsHDACs proteins change the structure of chromatin to increase or decrease the expression of a foreign gene in the genomes, so that the expression amount of the OsHDACs proteins can be controlled to produce a plant having varied phenotypic characteristics. Particularly, the OsHDAC1 protein is expressed locally in the plant and increases the growth rate of plant by its overexpression and its expression is increased by ABA. Therefore, this protein can be very efficiently used for producing plants having a high growth rate even under stress conditions including drought, cold, etc., as well as under normal conditions.
Posttranslational modification of histones, in particular acetylation and deacetylation are involved in the regulation of gene expression. Histone deacetylases remove acetyl groups from histone proteins. The present invention is directed to a method of regulating gene expression in a transgenic plant comprising, introducing into a plant a first chimeric nucleotide sequence comprising a first regulatory element in operative association with a coding sequence of interest, and an upstream activating sequence, and a second chimeric nucleotide sequence comprising a second regulatory element in operative association with a nucleotide sequence encoding histone deaceytlase and a nucleotide sequence encoding a DNA binding protein, and growing the transgenic plant. Furthermore, a method for regulating gene expression of an endogenous coding sequence of interest, or modifying a developmental, physiological or biochemical pathway in a plant is provided comprising introducing into a plant a chimeric nucleotide sequence comprising a regulatory element in operative association with a nucleotide sequence encoding histone deaceytlase fused with a nucleotide sequence encoding a DNA binding protein capable of interacting with an endogenous controlling sequence, for example an upstream activating sequence, and growing the transgenic plant. This invention also relates to novel histone deacetylase obtained from plants, to novel chimeric construct comprising these, or other histone deacetylase, and to transgenic plants, plant cells, or seeds comprising these chimeric constructs.
The present invention features substantially pure HDAC9, HDAC9a, HDAC9(.DELTA.NLS), HDAC9a(.DELTA.NLS), an HDRP(.DELTA.NLS) polypeptides, and isolated nucleic acid molecules encoding those polypeptides. The present invention also features vectors containing HDAC9, HDAC9a, HDAC9(.DELTA.NLS), HDAC9a(.DELTA.NLS), and HDRP(.DELTA.NLS) nucleic acid sequences, and cells containing those vectors.
The present invention features substantially pure HDAC9, HDAC9a, HDAC9(.DELTA.NLS), HDAC9a(.DELTA.NLS), an HDRP(.DELTA.NLS) polypeptides, and isolated nucleic acid molecules encoding those polypeptides. The present invention also features vectors containing HDAC9, HDAC9a, HDAC9(.DELTA.NLS), HDAC9a(.DELTA.NLS), and HDRP(.DELTA.NLS) nucleic acid sequences, and cells containing those vectors.
