Targeted molecular bar codes and methods for using the same are provided. The subject targeted molecular bar codes include a molecular bar code and a member of a specific binding pair, where the specific binding pair member is generally bonded to the bar code through a linking group. The subject molecular bar code may be read during translocation through a single nano-meter scale pore. The subject targeted molecular bar codes find use in a variety of different applications involving analyte detection, such as screening and diagnostic applications.
CROSS-REFERENCE TO RELATED APPLICATIONS
Pursuant to 35 U.S.C. .sctn.119 (e), this application claims priority to the filing date of the U.S. Provisional Patent Application Ser. No. 60/111,802 filed Dec. 11, 1998 and U.S. Provisional Patent Application Ser. No. 60/158,020 filed Oct. 6, 1999, the disclosures of which are herein incorporated by reference.
A plurality of particles of from about 5 nm to 100 .mu.m possessing predetermined isoelectric points in the pH range from about 2.5 to 11 is used in a method of detection of a plurality of analytes, wherein the isoelectric particles of each isoelectric point further contain a label and a member of a binding pair capable of interacting with a selected analyte. The particles that formed specific binding pairs are recovered and separated by isoelectric focusing, followed by the detection of the labels associated with the particles. A flow cytometer may be used as a detector of the isoelectric particles.
