Histone deacetylases and nucleotide sequences encoding said histone deacetylases are provided. The sequences as well as corresponding antisense constructs are useful for modulating gene activity in plants. Additionally, the sequences are useful for enhancing disease resistance in transformed plants.
CROSS-REFERENCE TO RELATED APPLICATION
This application is a divisional of U.S. application Ser. No. 09/282,305 filed Mar. 31, 1999, now issued as U.S. Pat. No. 6,287,843, and Provisional Application No. 60/080,563, filed Apr. 3, 1998, which is hereby incorporated herein in its entirety by reference.
Compounds that may be used to inhibit histone deacetylase having the formula Z-Q-L-M or Z-L-M wherein M is a substituent capable of complexing with a deacetylase catalytic site and/or a metal ion; L is a substituent providing between 0 10 atoms separation between the M substituent and the remainder of the compound; and Z and Q are as defined herein.
Histone deacetylase inhibitors and uses thereof are provided that have the general formula ##STR00001## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4 are each independently selected from the group consisting of hydrogen, a substituted or unsubstituted straight chained C.sub.1-12 alkyl, C.sub.2-12 aminoalkyl or C.sub.2-12 oxaalkyl, and a substituted and unsubstituted 3, 4, 5, 6, 7 or 8 membered ring, with the proviso that R.sub.3 and R.sub.4 are not both hydrogen; R.sub.5 is selected from the group consisting of a carbonyl, a substituted or unsubstituted C.sub.1-3 alkyl, a substituted or unsubstituted --C.sub.1-3 alkyl-C(O), a substituted or unsubstituted --C(O)--C.sub.1-3 alkyl, and a substituted or unsubstituted --C(O)C(O)C.sub.1-3 alkyl; M is a substituent capable of complexing with a protein metal ion; and L is a substituent comprising a chain of 3 12 atoms connecting the M substituent to the carbon atom alpha to the L substituent.
Compounds that may be used to inhibit histone deacetylase having the formula Z-Q-L-M or Z-L-M wherein M is a substituent capable of complexing with a deacetylase catalytic site and/or a metal ion; L is a substituent providing between 0-10 atoms separation between the M substituent and the remainder of the compound; and Z and Q are as defined herein.
Histone deacetylase inhibitors and uses thereof are provided that have the general formula ##STR00001## whereinR.sub.1, R.sub.2, R.sub.3 and R.sub.4 are each independently selected from the group consisting of hydrogen, a substituted or unsubstituted straight chained C.sub.1-12 alkyl, C.sub.2-12 aminoalkyl or C.sub.2-12 oxaalkyl, and a substituted and unsubstituted 3, 4, 5, 6, 7 or 8 membered ring, with the proviso that R.sub.3 and R.sub.4 are not both hydrogen;R.sub.5 is selected from the group consisting of a carbonyl, a substituted or unsubstituted C.sub.1-3 alkyl, a substituted or unsubstituted --C.sub.1-3 alkyl-C(O), a substituted or unsubstituted --C(O)--C.sub.1-3 alkyl, and a substituted or unsubstituted --C(O)C(O)C.sub.1-3 alkyl;M is a substituent capable of complexing with a protein metal ion; andL is a substituent comprising a chain of 3-12 atoms connecting the M substituent to the carbon atom alpha to the L substituent.
Compounds that may be used to inhibit histone deacetylase having the formula Z-Q-L-M or Z-L-M wherein M is a substituent capable of complexing with a deacetylase catalytic site and/or a metal ion; L is a substituent providing between 0-10 atoms separation between the M substituent and the remainder of the compound; and Z and Q are as defined herein.
