An immunochemical label comprising finely particulate carbon black on which is adsorptively immobilized a component which terminates distally from a point of adsorption with an immunologically active ligand or ligand binding molecule wherein said component comprises said immunological ligand or ligand binding molecules covalently linked through a linking reagent, and said linking reagent is adsorbed on the surface of said carbon black.
This application is a divisional of application Ser. No. 08/970,425, filed on Nov. 14, 1997, now U.S. Pat. No. 6,027,843 which is a Rule 60 divisional of Ser. No. 08/659,937, filed Jun. 7, 1996, now U.S. Pat. No. 5,728,587 which is a Rule 60 continuation of Ser. No. 08/070,803 now U.S. Pat. No. 5,559,041 filed Jun. 3, 1993; which is a Rule 60 divisional of Ser. No. 07/456,982 filed Dec. 18, 1989, now U.S. Pat. No. 5,252,496 the entire contents of which are hereby incorporated by reference.
The present invention concerns an immunological test for determining NT-proBNP comprising at least two antibodies to NT-proBNP, wherein at least one of the antibodies to NT-proBNP is a monoclonal antibody. One of these antibodies is directed at least against parts of the epitope of NT-proBNP comprising the amino acids 38 to 50. In addition, one of these antibodies is directed at least against parts of the epitope of NT-proBNP comprising the amino acids 1 to 37 or 43 to 76. The epitope recognized by the antibodies can slightly overlap.
