In vivo methods are provided for using an electric field to delivery therapeutic or immunizing treatment to a subject by applying non-invasive, user-friendly electrodes to the surface of the skin. Thus, therapeutic or immunizing agents can be delivered into cells of skin for local and systemic treatments or for immunization with optimal gene expression and minimal tissue damage. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.

The present invention relates to compositions for electroporation which are useful in elevating percutaneous absorbability of drugs, etc. By controlling electrolyte concentration of compositions for electroporation so as to make an osmotic pressure of the compositions not more than a physical osmotic pressure, the percutaneous absorbability of drugs can be elevated.

A minimally-invasive analyte detecting device and method for using the same. The system and method employ a device having an active electrode optionally coated with a substance, and a counter-electrode that is configured at least partially surround the active electrode. The configuration of the auxiliary electrode and active electrode improves the current flow through the device and increases the sensitivity of the device. When the device is placed against the patient's skin, the active electrode is adapted to enter through the stratum corneum of a patient to a depth less than a depth in the dermis at which nerve endings reside. An electric potential is applied to the active electrode and the analyte level is determined based on the amount of current or charge flowing through the device.

The present invention provides method for removal of body hair using electroporation using an apparatus having a pulse generator capable of generating electrical pulses of the desired amplitude and duration to kill hair follicles and an applicator having a first electrode placed in contact with a hair follicle and a second electrode interacting with the first electrode to provide an electroporating field in the tissue volume surrounding a hair to be removed. The method utilize a TENS system to provide patient relief from discomfort experienced by application of the electroporation pulses.

The present invention provides apparatus and method for removal of body hair using electroporation. The apparatus has a pulse generator capable of generator electrical pulses of the desired amplitude and duration to kill hair follicles and an applicator having a first electrode placed in contact with a hair follicle and a second electrode interacting with the first electrode to provide an electroporating field in the tissue volume surrounding a hair to be removed. The apparatus may include a TENS system to provide patient relief from discomfort experienced by application of the electroporation pulses.