Method for sequencing nucleic acid molecules - Patent Review 7056661

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Method for sequencing nucleic acid molecules

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United States Patent

7056661

Link to this page

http://www.wikipatents.com/7056661.html

Inventor(s)

Korlach; Jonas (Ithaca, NY), Webb; Watt W. (Ithaca, NY), Levene; Michael (Ithaca, NY), Turner; Stephen (Ithaca, NY), Craighead; Harold G. (Ithaca, NY), Foquet; Mathieu (Ithaca, NY)

Abstract

The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined.

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U.S. References

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7008766 Densham Mar,2006	Your vote accepted [0 after 0 votes]		6632655 Mehta et al. Oct,2003	Your vote accepted [0 after 0 votes]
2003/0186255 Williams et al. Oct,2003	Your vote accepted [0 after 0 votes]		2003/0194740 Williams et al. Oct,2003	Your vote accepted [0 after 0 votes]
2003/0064366 Hardin et al. Apr,2003	Your vote accepted [0 after 0 votes]		6524829 Seeger Feb,2003	Your vote accepted [0 after 0 votes]
6485944 Church et al. Nov,2002	Your vote accepted [0 after 0 votes]		2002/0164629 Quake et al. Nov,2002	Your vote accepted [0 after 0 votes]
6403311 Chan Jun,2002	Your vote accepted [0 after 0 votes]		6399335 Kao et al. Jun,2002	Your vote accepted [0 after 0 votes]
6355420 Chan Mar,2002	Your vote accepted [0 after 0 votes]		2002/0014850 Gilmanshin et al. Feb,2002	Your vote accepted [0 after 0 votes]
2002/0025529 Quake et al. Feb,2002	Your vote accepted [0 after 0 votes]		6306607 Williams et al. Oct,2001	Your vote accepted [0 after 0 votes]
6280939 Allen Aug,2001	Your vote accepted [0 after 0 votes]		6255083 Williams et al. Jul,2001	Your vote accepted [0 after 0 votes]
6263286 Gilmanshin et al. Jul,2001	Your vote accepted [0 after 0 votes]		6232075 Williams et al. May,2001	Your vote accepted [0 after 0 votes]
6210896 Chan Apr,2001	Your vote accepted [0 after 0 votes]		6221592 Schwartz et al. Apr,2001	Your vote accepted [0 after 0 votes]
6048690 Heller et al. Apr,2000	Your vote accepted [0 after 0 votes]		6027890 Ness et al. Feb,2000	Your vote accepted [0 after 0 votes]
6004744 Goelet et al. Dec,1999	Your vote accepted [0 after 0 votes]		5961923 Nova et al. Oct,1999	Your vote accepted [0 after 0 votes]
5922591 Anderson et al. Jul,1999	Your vote accepted [0 after 0 votes]		5858671 Jones Jan,1999	Your vote accepted [0 after 0 votes]
5846727 Soper et al. Dec,1998	Your vote accepted [0 after 0 votes]		5695934 Brenner Dec,1997	Your vote accepted [0 after 0 votes]
5703222 Grossman et al. Dec,1997	Your vote accepted [0 after 0 votes]		5688648 Mathies et al. Nov,1997	Your vote accepted [0 after 0 votes]
5677196 Herron et al. Oct,1997	Your vote accepted [0 after 0 votes]		5661028 Foote Aug,1997	Your vote accepted [0 after 0 votes]
5646264 Glazer et al. Jul,1997	Your vote accepted [0 after 0 votes]		5631134 Cantor May,1997	Your vote accepted [0 after 0 votes]
5620854 Holzrichter et al. Apr,1997	Your vote accepted [0 after 0 votes]		5601982 Sargent et al. Feb,1997	Your vote accepted [0 after 0 votes]
5547835 Koster Aug,1996	Your vote accepted [0 after 0 votes]		5470710 Weiss et al. Nov,1995	Your vote accepted [0 after 0 votes]
5403708 Brennan et al. Apr,1995	Your vote accepted [0 after 0 votes]		5405747 Jett et al. Apr,1995	Your vote accepted [0 after 0 votes]
5302509 Cheeseman Apr,1994	Your vote accepted [0 after 0 votes]		5200313 Carrico Apr,1993	Your vote accepted [0 after 0 votes]
4994373 Stavrianopoulos et al. Feb,1991	Your vote accepted [0 after 0 votes]
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0745686Dec., 1996EP	Your vote accepted [0 after 0 votes]		0 258 017Jun., 1997EP	Your vote accepted [0 after 0 votes]
0 834 576Apr., 1998EP	Your vote accepted [0 after 0 votes]		WO 90/13666Nov., 1990WO	Your vote accepted [0 after 0 votes]
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