DNAs encoding human voltage gated sodium channel .beta.1A subunit have been cloned and characterized. The recombinant protein is capable of forming biologically active protein. The cDNA's have been expressed in recombinant host cells that produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the receptor activity, and receptor modulators are identified.
This case is a divisional application of U.S. patent application Ser. No. 09/875,456 filed Jun. 6, 2001 and entitled "The Human Voltage Gated Sodium Channel .beta.1A Subunit and Method of Use" which claims priority from U.S. Provisional Patent Application No. 60/294,405 filed Jun. 7, 2000 and entitled "DNA Encoding Human Voltage Gated Sodium Channel .beta.1A Subunit" and U.S. Provisional Patent Application No. 60/236,664 filed Sep. 29, 2000 also entitled "DNA Encoding Human Voltage Gated Sodium Channel .beta.1A Subunit" the contents of which are all hereby incorporated by reference in their entirety.
Disclosed are high throughput assay systems and methods for identifying agents that alter the level of surface expression of integral membrane proteins, such as cardiac ion channels, in mammalian cells. Also disclosed are therapeutic methods of using agents identified using such methods.
