Disclosed are improved methods for creating a uniform vascular wound in a zebrafish larva or zebrafish. The methods illustratively include subjecting a zebrafish larva to laser irradiation in an amount and for a period of time effective to cause a uniform vascular wound in the zebrafish larva; or exposing a zebrafish to water containing sodium hydroxide in an amount and for a period of time effective to cause a uniform vascular wound detectable in the gills of the zebrafish.
The present application is a United States nationalization of PCT International Patent Application PCT/US2003/041249, filed Dec. 24, 2003, which claims priority to first U.S. provisional application Ser. No. 60/436,270, filed Dec. 24, 2002, and to second U.S. provisional application Ser. No. 60/456,774, filed Mar. 21, 2003, the entire disclosures of which applications are specifically incorporated herein by reference without disclaimer.
