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| United States Patent | 7364851 |
| Link to this page | http://www.wikipatents.com/7364851.html |
| Inventor(s) | Berlin; Andrew (San Jose, CA), Kirch; Steven J. (Pleasanton, CA), Neubauer; Gabi (Los Gatos, CA), Rao; Valluri (Saratoga, CA), Yamakawa; Mineo (Campbell, CA) |
| Abstract | The methods, compositions and apparatus disclosed herein are of use for
nucleic acid sequence determination. The methods involve isolation of one
or more nucleic acid template molecules and polymerization of a nascent
complementary strand of nucleic acid, using a DNA or RNA polymerase or
similar synthetic reagent. As the nascent strand is extended one
nucleotide at a time, the disappearance of nucleotide precursors from
solution is monitored by Raman spectroscopy or FRET. The nucleic acid
sequence of the nascent strand, and the complementary sequence of the
template strand, may be determined by tracking the order of incorporation
of nucleotide precursors during the polymerization reaction. Certain
embodiments concern apparatus comprising a reaction chamber and detection
unit, of use in practicing the claimed methods. The methods, compositions
and apparatus are of use in sequencing very long nucleic acid templates
in a single sequencing reaction. |
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Title Information  |
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| Publication Date |
April 29, 2008 |
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| Parent Case |
This application is a divisional application of U.S. Ser. No. 09/962,288,
filed Sep. 24, 2001, now U.S. Pat. No. 6,852,492, issued Feb. 8, 2005.
The disclosure of the prior application is considered part of and is
incorporated by reference in the disclosure of this application. |
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