Disclosed are enzyme assays using biolayer interferometry. Assays may be carried out using immobilized substrate or with a substrate capture format. In certain embodiments, the assays are carried out using unlabeled substrates. The methods are broadly applicable to enzyme assay measurements, can be carried out in vivo or in vitro, and are easily multiplexed.
CROSS REFERENCE TO RELATED APPLICATIONS
The present application claims the benefit of U.S. Provisional Application Ser. No. 60/645,153, filed Jan. 19, 2005 and U.S. Provisional Application Ser. No. 60/642,454, filed Jan. 7, 2005, both of which are incorporated herein by reference in their entirety for all purposes.
