There is provided a substrate for lysing cells and purifying nucleic acid having a matrix and a coating and an integrity maintainer for maintaining the purified nucleic acid. Also provided is a method of purifying nucleic acid by applying a nucleic acid sample to a substrate having an anionic detergent affixed to a matrix, the substrate physically capturing the nucleic acid, bonding the nucleic acid to a substrate and generating a signal when the nucleic acid bonds to the substrate indicating the presence of the nucleic acid. A kit for purifying nucleic acid containing a coated matrix and an integrity maintenance provider for preserving the matrix and purifying nucleic acid is also provided.
CROSS-REFERENCE TO RELATED APPLICATIONS
This is a continuation application of U.S. patent application Ser. No. 09/736,659, filed 14 Dec. 2000 now U.S. Pat. No. 6,746,841, which is a continuation-in-part of PCT application No. PCT/US00/10230, filed Apr. 14, 2000, which claims the benefit of priority under 35 USC Section 119(e) of U.S. Provisional Patent Application No. 60/129,191, filed on Apr. 14, 1999; U.S. Provisional Patent Application No. 60/180,353, filed on Feb. 4, 2000; and U.S. Provisional Patent Application No. 60/193,556, filed on Mar. 31, 2000, all of which are incorporated herein by reference.
