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Intraplexing method for improving precision of suspended microarray assays
   
Document Number
US Patent 7501290
Issued Date
March 10, 2009
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Abstract
A method for making suspended microarray readings from a single sample statistically significant in a reliable manner. It can be applied to any assay system that uses discrete particles where the particles are coupled with an assay. Most of these systems suspend the particles in fluid and read the assay result using flow cytometry. However, other methods such as the distribution of tiny assay devices coupled with miniature transponders, where the sampling is of the environment, can also make use of this method. This invention combines multiple separately identified assays of one sample, where the multiple assays are for a single analyte and sets of assays have differing sensitivity to said analyte. By elimination of outliers, averaging, and taking ratios of averages, this method makes possible highly reliable diagnostics. This method compensates for multiple stochastic and non-stochastic sources of errors that can occur in this type of assay system.
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Number of Claims:
17
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Published
March 10, 2009
Application Number
11/901,117
Filed
September 13, 2007
US Classification
436/518  
Int'l Classification
G01N   33/543   (20060101)  
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Parent Case
RELATED APPLICATION This application claims priority from the U.S. provisional application with Ser. No. 60/844,577, which was filed on Sep. 13, 2006. The disclosure of that provisional application is incorporated herein as if set out in full.
USPTO Field of Search
436/518   436/523   435/7.92  
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