The present invention discloses improved methods of disaggregating protein aggregates, and refolding denatured proteins, using high pressure. In particular, the present invention provides for the use of agitation, high temperature, "stepped" depressurization, dialysis and dilution under pressure to increase the speed and extent of aggregate dissolution and protein refolding.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of PCT/US01/45728, filed Oct. 31, 2001, which claims priority from U.S. Provisional Application Ser. No. 60/244,808, filed Oct. 31, 2000.
