Conjugates of isomaleimides and isophthalimides and organic chromophoric compounds exhibiting fluorescence, luminescence, chemiluminescence or absorption of analytical value having the structural formula I and II ##STR1## wherein R is an organic aromatic molecule which may be substituted, heteroaromatic which may be substituted, aliphatic having 1 to 50 carbon atoms and which may be substituted or an organic chromophoric molecule exhibiting fluorescence, luminescence, chemiluminiescence or absorption of analytical value. R' is an organic chromophoric molecule exhibiting fluorescence, luminescence, chemiluminescence, or absorption of analytical value; R.sub.1 is hydrogen, halogen, carboxylic, alkyl, aryl, hydroxyl, amino group which may be substituted, nitro or sulfonic group. R.sub.2, R.sub.3 and R.sub.4 have the same meaning as R.sub.1 ; X is hydrogen, alkyl, aryl, halogen, carboxylic, alkoxy, aryloxy, hydroxyl, amino group which may be substituted or alkyl group forming a five or six member ring with the adjacent olefinic carbon atom; Y has the same meaning as X; Z is hydrogen, alkyl having 1 to 18 carbon atoms or aryl. The maleic diamides and the phthalic diamides of the invention may be used for the labeling of organic substrates as staining materials and as indicators.
Novel adducts of oxazine urea chromophors or thiazine urea chromophors with organic substrates are provided which are useful in analytical techniques for the detection and measurement of biological and clinical compounds of interest.
The invention relates to oxazine derivatives by means of which a broad spectrum of material to be examined can be marked and identified using fluorescence techniques.
