A marking syringe which allows an individual using the syringe to inject a fluid such as a vaccine into an animal and, at the same time, mark the location of the injection on the animal. More specifically, the marking syringe includes a vaccine syringe and an ink syringe connected to a handle. Activation of the handle simultaneously activates the vaccine syringe and the marking syringe. The vaccine syringe and the ink syringe are respectively connected to a source of vaccine and a source of ink. After connection to the vaccine and ink sources, the syringe needle is inserted into the animal and a syringe handle is actuated. As the handle is actuated, a vaccine syringe and an ink syringe both discharge their contents. The position of the ink syringe relative to the vaccine syringe is such that the discharged ink marks the animal in the approximate location of the vaccine injection.

A marking syringe which allows an individual using the syringe to inject a fluid such as a vaccine into an animal and, at the same time, mark the location of the injection on the animal. More specifically, the marking syringe includes a vaccine syringe and an ink dispenser connected to a handle. Activation of the handle simultaneously activates the vaccine syringe and the ink dispenser. The vaccine syringe is connected to a source of vaccine and a source of ink is provided. After connection of the vaccine and ink sources, the syringe needle is inserted into the animal and a syringe handle is actuated. As the handle is actuated, a vaccine syringe and an ink dispenser both discharge their contents. The position of the ink dispenser relative to the vaccine syringe is such that the discharged ink marks the animal in the approximate location of the vaccine injection.

A loading station having a dispensing manifold for loading syringe pairs with thrombin and adhesive and clotting proteins for use as a biological adhesive. The resulting loaded syringe pairs are compatible with a variety of biological adhesive dispensers and may be used in a surgical setting.

An improved barrier or drug delivery system which is highly adherent to the surface to which it is applied is disclosed, along with methods for making the barrier. In the preferred embodiment, tissue is stained with a photoinitiator, then the polymer solution or gel having added thereto a defined amount of the same or a different photoinitator is applied to the tissue. On exposure to light, the resulting system polymerizes at the surface, giving excellent adherence, and also forms a gel in the rest of the applied volume. Thus a gel barrier of arbitrary thickness can be applied to a surface while maintaining high adherence at the interface. This process is referred to herein as "priming". The polymerizable barrier materials are highly useful for sealing tissue surfaces and junctions against leaks of fluids. In another embodiment, "priming" can be used to reliably adhere preformed barriers to tissue or other surfaces, or to adhere tissue surfaces to each other. A first surface and a barrier, or another surface, are prestained with initiator, and a thin layer of gellable monomer containing initiator is placed between them. Strong adhesion is obtained between the two surfaces on gelation of the monomer. In a similar fashion, tissue surfaces can be adhered to each other in repair of wounds and formation of anastomoses. Methods for use of non-photochemical systems and combined chemical/photochemical systems are described.

An applicator for dispensing a first and second component of a biological adhesive, such as fibrin glue. At least one of said components may contain a suspension of fibrin microbeads (FMB) or a suspension of cells. The present invention uses a single supply of pressurized gas to force the components from the applicator using positive fluid pressure and to atomize them into a convergent spray. Another embodiment of the present invention also provides for the endoscopic application of the biological adhesive directly to tissue defects. The application of positive pressure allows precise metering of the components and application of the adhesive, prevents internal coagulation of the fibrin or clogging by suspended particles and reduces waste and contamination of the components.