A modified bioluminescent protein responds to different physical, chemical, biochemical or biological conditions to product light or radiation of altered characteristics when the bioluminescent reaction is initiated. The modified bioluminescent protein may respond to modification thereof, e.g. by covalent modification. The protein may include signal peptides to "target" it. DNA coding for the bioluminescent protein may be altered to include tissue specific promoter or enhancer genes so that the altered DNA acts as reporter gene.
This application is a division of application Ser. No. 08/270,314, filed Jul. 5, 1997, now U.S. Pat. No. 5,683,888 which is a file wrapper continuation of Ser. No. 07/820,867, filed Jan. 22, 1992 now abandoned.
A modified bioluminescent system comprising a fluorescent molecule covalently linked with a photoprotein, wherein said link between the two proteins has the function to stabilize the modified bioluminescent system and allowing the transfer of the energy by Chemiluminescence Resonance Energy Transfer (CRET).
