The present invention relates to a composition suitable for use in an ECL assay wherein electromagnetic radiation emitted by the composition is detected. The composition includes an amine or amine moiety which, when oxidized by exposure to an effective amount of electrochemical energy, forms a strong reducing agent in the composition; and an electrolyte capable of functioning as a medium in which an ECL moiety and the amine or amine moiety can be oxidized by exposure to electrochemical energy.
This application is a continuation of application Ser. No. 08/467,936, filed Jun. 6, 1995, now U.S. Pat. No. 6,271,041, which is a division of application Ser. No. 08/196,315, filed Apr. 15, 1994, now U.S. Pat. No. 6,165,729, which is a continuation of application Ser. No. 07/266,914, filed Nov. 3, 1988, now abandoned, which is a continuation-in-part of application Ser. No. 07/188,258, filed Apr. 29, 1988, now abandoned. Application Ser. No. 07/188,258 was refiled as application Ser. No. 07/570,226, filed Aug. 21, 1990, abandoned, which was refiled as application Ser. No. 07/773,971, filed on Sep. 27, 1991, now U.S. Pat. No. 5,147,806, all of which are incorporated herein by reference.
