A method of using carboxyfluorescein in ophthalmic studies comprising applying of the eye an effective amount of at least one active isomer of carboxyfluorescein.

The present invention is directed to a non-isotopic methods for the in vitro and in vivo detection of hydroxyapatite-positive cells and structures.

A fluorescent agent for monitoring organ function, such as glomerular filtration, renal blood flow, or hepatic function. The agent is injected into a subject and the fluorescence monitored in vivo via time-resolved fluorescent techniques. The agent is a lanthanide ion chelated to a polyaminopolyacetic acid analog. A new clearance agent is also proposed based on a tetraazamacrocycle. Such a clearance agent also finds applications in other fields where fluorescence detection is exploited.

A method and apparatus for measuring the heterogeneity of oxygen delivery to perfused and in situ organs. A laser is employed as a flying spot scanning excitation fluorometer source which provides excitation for either oxidized flavoprotein of the mitochondrial space or reduced nucleotide of mitochondrial and cytosolic spaces. Emission from the two fluorochromes is acquired at 10.sup.4 to 10.sup.5 data points per sec. and histograms of the fluorescence intensity versus the number of occurrences ...

Fluorescent chelates of terbium and europium with tri- and tetra-cyclopolyazamacrocyclic compounds are discussed which can be used as fluorescent in vitro or in vivo diagnostic agents. These chelates are tissue specific imaging agents.

The present invention is used for diagnosis, including early diagnosis, of malignant growths, for assessment as of the extent of malignant infection, for determining of the required volume for surgical intervention, for finalizing diagnoses under clinical and dispensary conditions, for control during the process of treatment of malignant tumors, and for detection of post-operative relapses. The contrasting composition according to the present invention is characterized in that, in addition to th...

An ophthalmic composition for use in the human eye comprising an aqueous solution of fluorexon whose pH has been adjusted to between pH 5.4 and 6.2 to deliver maximum fluorescence and additionally containing sufficient buffer to maintain that pH even after instillation into the eye.

Kits containing the diagnostic systems and diagnostic systems that rely on bioluminescence for visualizing tissues in situ are provided. The systems include compositions containing conjugates that include a tissue specific, particularly a tumor-specific, targeting agent linked to a targeted agent, a luciferase or luciferin. The systems also include a second composition that contains the remaining components of a bioluminescence generating reaction. Administration of the compositions results prod...

The present invention relates to the use of particulate materials as contrast agents in in vivo light imaging.

The present invention relates to novel fluorescent dyes, novel fluorogenic and fluorescent reporter molecules and new enzyme assay processes that can be used to detect the activity of caspases and other enzymes involved in apoptosis in whole cells, cell lines and tissue samples derived from any living organism or organ. The reporter molecules and assay processes can be used in drug screening procedures to identify compounds which act as inhibitors or inducers of the caspase cascade in whole cell...