A plurality of particles of from about 5 nm to 100 .mu.m possessing predetermined isoelectric points in the pH range from about 2.5 to 11 is used in a method of detection of a plurality of analytes, wherein the isoelectric particles of each isoelectric point further contain a label and a member of a binding pair capable of interacting with a selected analyte. The particles that formed specific binding pairs are recovered and separated by isoelectric focusing, followed by the detection of the lab...

Antibodies to serum hepatitis are coupled to carbohydrate macro particles which are then contacted with blood fluids, suitably plasma, which are believed to contain serum hepatitis antigen. The serum hepatitis antigen is thus complexed with the bound antibody and the particles bearing both the antibody and the antigen are removed from the blood fluid. The antigen is then cleaved from the antibody by alkaline treatment, and may, if desired, be itself isolated, suitably by dialysis. The antibody/a...

Immunoassay technique employing sacs having a lysable membrane and at least one determinant or epitopic site recognizable by an antibody. Enclosed in the sacs are water soluble stable free radical compounds, which are capable of escape from the sac upon lysis. The immunoassay can be employed for the determination of an epitope containing compound, antibodies or complement. Two basic modes are employed. The first mode depends upon the difference in the EPR spectrum of the stable free radical when...

An analytical technique for the accurate and precise measurement of trace water in chemical reagents, comprising the steps of combining a chemical reagent comprising .ltoreq.5 ppm water, with hexafluoroacetone (HFA), to form a sample mixture comprising at least the chemical reagent and a water derivative of hexafluoroacetone; and measuring the concentration of the water derivative of hexafluoroacetone by gas chromatography.

An emulsion (in which an aqueous medium contains small liquid droplets coated with a protein that will interact specifically with a select protein) is mixed with a liquid sample, time is allowed for interaction to occur and the mixture is then exposed to a tagged antibody specific to the select protein. After an appropriate hold time the emulsion is broken and the protein that previously covered the disperse droplets becomes concentrated at the surface. The surface is checked for the presence of...

A latex agglutination process for the detection and determination of a partner of an antigen-antibody reaction is described, wherein an antigen and an antibody are reacted in the presence of a gamma globulin containing no antibodies specific for the antigen.

A new diagnostic labeled spine tool is disclosed for use in immunoassay techniques, e.g., determination of a component of the antigen-antibody reaction. The new tool is a labeled-spine material product of the formula ##STR1## where R is an organic group labeled with a radioisotope, fluorescent group, lysis-initiating compound, enzyme, or other suitable marker material; T.sub.1 and T.sub.2 are any of --CH.sub.3, ##STR2## --CH.sub.2 OH, --Ch.sub.2 SH, and --NH.sub.2 ; X is selected from the group ...

The present invention provides imprint compositions useful for capturing, isolating, detecting and/or quantifying macromolecules in a sample, methods of making and using the same. Generally, the imprint compositions comprise a matrix material defining an imprint of a template molecule, and the template molecule typically corresponds to a portion of a macromolecule of interest.

Chemiluminescent substrate delivery systems comprising a conjugate of a dendrimer and at least one chemiluminescent substrate are provided. The substrate delivery systems can also include a chemiluminescence enhancer. The dendrimer/chemiluminescent substrate conjugates can be used in kits including an enzyme capable of activating the chemiluminescent substrate to produce a peroxygenated intermediate that decomposes to produce light. The dendrimer/chemiluminescent substrate conjugates can be used...

Liquid containing hepatitis A antibody is adsorbed on a surface. The surface is then coated with a proteinaceous material and incubated in the presence of the sample to form a complex of hepatitis A antigen and hepatitis A antibody. The complex is incubated again in the presence of excess radioactively labelled hepatitis A antibody, and the resulting radionuclide is measured.