A conjugate for use in the detection and quantification of antibodies and antigens in body fluids by immunoassay procedures and chemiluminescent immunoassay procedures utilizing the conjugate. The conjugate is capable of reacting with an antigen or an antibody or both and includes a tag capable of catalyzing a chemiluminescent reaction. The conjugate may be an antibody or an antigen to which a metallo porphyrin tag is attached and preferably comprises immunoglobulin to which hemoglobin is attach...

Biological fluids, such as blood sera, are placed individually on a testing surface like an acrylic coating alongside or in between control sera. The coating has an affinity for nonagglutinated particles. To each of the serum areas a drop of testing reagent is added and mixed. The mixtures are allowed to incubate or mature for approximately 5 to 10 minutes. An absorbent material is then applied to the test surface to remove the agglutinated particles of the mixtures from the test surface. The te...

There is provided a novel composition for the direct determination of the existence of pregnancy by the admixture therewith of a sample of urine, plasma, or serum from the female believed to be pregnant. This novel composition is prepared by mixing serum obtained from rabbits previously injected with human chorionic gonadotrophin (HCG), with polystyrene latex particles and heating for a short time.

An ignitable charge for producing pressurized gas to power industrial tools, that is made of plentiful, common materials at a very low cost, lower in cost than conventional charges by orders of magnitude. The charge is comprised of a hollow plastic jacket of triangular cross section that is open through its center to both opposite ends and contains a nitrocellulose propellant in uncompressed, or expanded form that may be ignited through one end of the jacket by a spark, hot wire, or the like in ...

A method of immobilizing aminated DNA to a substrate surface is provided for use in DNA hybridization assays. The surface can be widely employed in a variety of molded laboratory products including multiwell plates, cell culture dishes, and biological containers. The method comprises the steps of providing a polystyrene substrate having a surface content of between 0.3 and 4.0% atomic percent oxygen, and attaching amine modified oligonucleotide to the surface of the substrate. A resultant produc...

A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a chromatographic zone on which is disposed a plurality of microporous particles. The chromatographic zone can effectively reduce the "hook effect" in a simple, efficient, and relatively inexpensive manner. In particular, the plurality of microporous particles allows larger-sized analyte/probe complexes to reach the detection zone before the uncomplexed an...

A chromatographic method and apparatus for serum thyroxine iodine determination including sealed ion exchanged resin column structures which are opened and press fit and sealed to funnel members for use and a method in which serum protein is removed from resin bound thyroxine in alkaline solution, with a pH of substantially 8.

A test for determining the anti-coagulation level in blood samples is based on ascertaining the presence of heparin-antithrombin-III complex therein. This may be done with the aid of antibodies against thrombin-antithrombin-III complex, such antibodies being used e.g. in the form of a latex reagent or a bloodcell reagent.

Method for the determination of thyroxine in biological fluids by adsorption on a cation exchanger, subsequent washing out of the interfering components, eluting the adsorbed thyroxine, liberating the iodine therefrom, and then carrying out the iodine determination in a conventional manner, which process is characterized by the use of an eluent, optionally together with a basic buffer, which destroys amino groups and liberates iodine from the thyroxine into the eluate.

An immobilized hapten reagent for use in a specific binding assay for the determination of a hapten or binding analog thereof in a liquid test sample and methods for preparing and using the immobilized hapten reagent. The immobilized hapten reagent comprises a hapten moiety covalently linked substantially only to the external surface of a polyacrylamide gel particle. The immobilized hapten reagent is prepared by forming a reaction mixture comprising the hapten moiety and the carrier material in ...