
To determine iron in serum by the release of bound iron and reduction of the released iron to Fe.sup.2+, a first solution of a denaturing agent at a concentration of 1 to 8 mol/l is added to the serum, then a second solution is added which contains the color reagent and likewise a denaturing agent at a concentration of 1 to 8 mol/l and subsequently the colored complex is measured photometrically.











