Methods and compositions are provided for gene transfer to intact mammals with expression of the exogenous genetic material in the host. Mammalian host cells which are regenerative, normally highly proliferative or subject to induced proliferation, are transformed or modified in vitro with DNA capable of replication and expression in the host cell, wherein the DNA becomes incorporated into the cell. The modified cells are found to regenerate in the host with expression of the introduced DNA. Par...

Methods and compositions are provided for gene transfer to intact mammals with expression of the exogenous genetic material in the host. Mammalian host cells which are regenerative, normally highly proliferative or subject to induced proliferation, are transformed or modified in vitro with DNA capable of replication and expression in the host cell, wherein the DNA becomes incorporated into the cell. The modified cells are found to regenerate in the host with expression of the introduced DNA. Par...

A combination shoe, lace and new merchandise indicia and lace retainer, the shoe being fully or substantially fully laced, and the new merchandise indicia and lace retainer having a central portion looped around doubled end portions of the lace and having overlapping end portions secured together. The doubled end portions of the lace and the new merchandise indicia and lace retainer are tucked into the foot inlet of the shoe during storage and are pulled out of the foot inlet for trial fit of th...

System, method and apparatus for carrying out the recovery of an intact volume of tissue wherein a delivery cannula distal end is positioned in confronting adjacency with the volume of tissue to be recovered. An expandable metal capture component is expressed from the distal end of the cannula to expand while being electrically excited to electrosurgically cut around and circumscribe the tissue volume. Pursing cables are tensioned to complete the envelopment of the tissue volume, whereupon the v...

System, method and apparatus for carrying out the recovery of an intact volume of tissue wherein a delivery cannula distal end is positioned in confronting adjacency with the volume of tissue to be recovered. A capture component formed of a plurality of metal leafs is deployed from the distal end of the delivery cannula. The tips of these leafs carry a pursing cable assembly which is electrically excited to electrosurgically cut around and circumscribe the tissue volume. These pursing cables are...

A rake (10) for removing weeds (12) with intact roots (14) from the soil (16) comprising an elongate handle (18). A spine (20) has a plurality of tines (22) extending therefrom. A structure (24) is provided for attaching the spine (20) traversely to the elongate handle (18). A crosspiece (26) is provided. A facility (28) is provided for affixing the crosspiece (26) across a rear surface (30) of the tines (22) at a predetermined distance "A" from distal free ends (32) of the tines (22). When the ...

The present invention relates to novel methods of determining the sequence and structure of proteins. Specifically, the present invention allows for the analysis of intact proteins within a mass spectrometer. Therefore, preparatory separations need not be performed prior to introducing a protein sample into the mass spectrometer. Also disclosed herein are new instrumental developments for enhancing the signal from the desired modified proteins, methods for producing controlled protein fragments ...

The present invention relates to an automated method of quantifying DNA strand breaks in intact cells whereby (a) cells are lysed in order to release DNA, (b) the lysate is subjected to an alkaline denaturation process, (c) neutralization occurs, (d) a fluorescent dye is added, and (e) fluorescence is read off a fluorometer, and the method is characterized in that all pipetting stages are carried out in automated fashion by means of a pipetting device in a lightproof housing. The invention furth...

The invention provides erythrocytes of improved O.sub.2 -release properties having fused thereto small allosteric effectors, f.i. inositol hexaphosphate-containing lipid vesicles. Also included in the invention is a method for making said erythrocytes and plasma containing said erythrocytes.

A method and apparatus is provided which will attenuate bioelectric interference when measuring the activity in an intact nerve. An electrode probe is provided having first and second electrodes positioned on opposite sides of a thin, flexible, insulating carrier. The probe is inserted between the nerve to be measured and the underlying body tissue with one electrode contacting only the nerve and the second electrode contacting only the tissue. The nerve and the tissue thereby form in effect a W...